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Resveratrol directly affects in vitro lipolysis and glucose transport in human fat cells

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Abstract

Resveratrol is a naturally occurring polyphenol found in many dietary sources and red wine. Recognized as a cancer chemoprevention agent, an anti-inflammatory factor and an antioxidant molecule, resveratrol has been proposed as a potential anti-obesity compound and to be beneficial in diabetes. Most of the studies demonstrating the anti-adipogenic action of resveratrol were performed as long-term treatments on cultured preadipocytes. The aim of this study was to analyse the acute effects of resveratrol on glucose uptake and lipolysis in human mature adipocytes. Samples of subcutaneous abdominal adipose tissue were obtained from overweight humans and immediately digested by liberase. Fat cells were incubated (from 45 min to 4 h) with resveratrol 1 μM–1 mM. Then, glycerol release or hexose uptake was determined. Regarding lipolysis, the significant effects of resveratrol were found at 100 μM, consisting in a facilitation of isoprenaline stimulation and an impairment of insulin antilipolytic action. At 1 and 10 μM, resveratrol only tended to limit glucose uptake. Resveratrol 100 μM did not change basal glucose uptake but impaired its activation by insulin or by benzylamine. This inhibition was not found with other antioxidants. Such impairment of glucose uptake activation in fat cells may led to a reduced availability of glycerol phosphate and then to a decreased triacylglycerol assembly. Therefore, resveratrol increased triacylglycerol breakdown triggered by β-adrenergic activation and impaired lipogenesis. Consequently, our data indicate that resveratrol can be considered as limiting fat accumulation in human fat cells and further support its use for the mitigation of obesity.

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Acknowledgments

This work was partly supported by Communauté de Travail des Pyrénées and the DIOMED project (INTERREG IVB-SUDOE-FEDER, SOE1/P1/E178). The authors express gratitude to Simon Rascalou, Sarah Canteiro and Sandra Grès for their help. They also acknowledge Philippe Valet (Univ. Paul Sabatier, Toulouse, France) and Maria P. Portillo (Univ. of Pais Vasco, Vitoria, Spain) for their respective knowledge on human adipocyte biology and dietary diphenolic compounds and the staff of Plastic Surgery Dpt. of Rangueil Hospital for facilitating access to their post-surgical wastes.

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Authors and Affiliations

  1. I2MC, Université de Toulouse, UPS, Toulouse, France

    Saioa Gomez-Zorita

  2. Institut des Maladies Métaboliques et Cardiovasculaires, Institut National de la Santé et de la Recherche Médicale (INSERM U1048), 31432, Toulouse Cedex 4, France

    Karine Tréguer, Josep Mercader & Christian Carpéné

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  1. Saioa Gomez-Zorita
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  2. Karine Tréguer
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  3. Josep Mercader
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  4. Christian Carpéné
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Correspondence to Christian Carpéné.

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Submitted to Journal of Physiology and Biochemistry in 2012

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Gomez-Zorita, S., Tréguer, K., Mercader, J. et al. Resveratrol directly affects in vitro lipolysis and glucose transport in human fat cells. J Physiol Biochem 69, 585–593 (2013). https://doi.org/10.1007/s13105-012-0229-0

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  • DOI: https://doi.org/10.1007/s13105-012-0229-0

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