Abstract
Random Amplified Polymorphic DNA (RAPD) markers were utilized for determination of diversity within and among the three populations of Nepenthes khasiana Hook f., a threatened insectivorous plant of Meghalaya (India). A total of 90 bands were generated from 10 random amplification polymorphic DNA (RAPD) primers of which 71 were found to be polymorphic (78.89%). Nei’s gene diversity (h) ranged between 0.124–0.201 with overall diversity of 0.228 while Shannon’s information index I) values recorded between 0.187–0.308 with an average value of 0.352. The values of gene flow (Nm = 1.284) and the diversity among populations (0.280) recorded demonstrates higher genetic variation within the population. AMOVA analysis revealed a low level of genetic variation (21.96%) among the populations. This study indicates that some variation still exists within and between the existing populations of N. khasiana, thus, these populations could provide materials for re-establishing of this important rare and threatened species.
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References
Bhau BS, Medhi K, Sarkar T, Saikia SP. 2009. PCR based molecular characterization of Nepenthes khasiana Hook. f.-pitcher plant. Genet. Resour. Crop Evol. 56: 1183–1193
Bordoloi RPM. 1977. The pitcher plant Nepenthes khasiana. Sreeguru press, Guwahati
Charlesworth B, Nordborg M, Charlesworth D. 1997. The effects of local selection, balanced polymorphism, and back ground selection on equilibrium patterns of genetic diversity in subdivided populations. Genet. Res. 70: 155–174
Chaveerach A, Tanomtang A, Sudmood R, Tanee T. 2006. Genetic diversity among geographically distributed population of Nepenthes mirabilis. Biologia (Bratisl) 61: 295–298
Excoffier L, Laval G, Schneider S. 2005. Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evol. Bioinform. Online 1: 47–50
Hamrick JL, Godt MJW. 1989. Allozyme diversity in plant species. In AHD Brown, MT Clegg, AL Kahler, BS Weir, eds, Plant Population Genetics, Breeding and Genetic Resources, Sinauer, Sunderland, Massachusetts, pp 43–63
Hogbin PM, Peakall R. 1999. Evaluation of the conservation of genetic research to the management of endangered plant Zieria prostrata. Conserv. Biol. 13: 514–522
Jebb M, Cheek M. 1997. A skeletal revision of Nepenthes (Nepenthaceae). Blumea 42: 86–87
Kimura M, Crow J. 1964. The number of alleles that can be maintained in a finite population. Genetics 49: 725–738
Kumar S, Kumaria S, Sharma SK, Rao SR, Tandon P. 2011. Genetic diversity assessment of Jatropha curcas L. germplasm from Northeast India. Biomass Bioenerg. 35: 3063–3070
Lewontin RC. 1972. The apportionment of human diversity. Evol. Biol. 6: 381–398
Lynch M, Milligan BG. 1994. Analysis of population structure with RAPD markers. Mol. Ecol. 3: 91–99
Meffe GK. 1994. Genetics: conservation of diversity within species. In GK Meffe, CR Carroll, eds, Principles of Conservation Biology. Sinauer Associates, Sunderland, pp 161–201
Miller MP. 1998. AMOVA-PREP1.01. A Program for the Preparation of AMOVA Input Files from Dominant-Marker Raw Data. Northern Arizona University, Flagstaff, AZ. USA
Nei M. 1973. Analysis of gene diversity in subdivided populations. Proc. Nat. Acad. Sci. USA 70: 3321–3323
Porebski S, Bailey LG, Baum BR. 1997. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol. Biol. Rep. 15: 8–15
Prevost A, Wilkinson MJ. 1999. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor. Appl. Genet. 98: 107–112
Rohlf FJ. 1998. NTSYSpc Numerical Taxonomy and Multivariate Analysis System, Version 2.02. Setauket, Exeter Software
Savolainen O, Pyhajarvi T, Knurr T. 2007. Gene flow and local adaptation in trees. Annu. Rev. Ecol. Evol. Syst. 38: 595–619
Schoen DJ, Brown AHD. 1991. Intraspecific variation in population gene diversity and effective population size correlates with the mating system in plants. Proc. Natl. Acad. Sci. USA 88: 4494–4497
Sharma SK, Kumar S, Rawat D, Kumaria S, Kumar A, Rao SR. 2011. Genetic diversity and gene flow estimation in Prosopis cineraria (L.) Druce: A key stone tree species of Indian Thar Desert. Biochem. Syst. Ecol. 39: 9–13
Wright SI, Gaut BS. 2005. Molecular population genetics and the search for adaptive evolution in plants. Mol. Biol. Evol. 22: 506–519
Yeh FC, Young RC, Boyle T. 1999. Microsoft Window-based Freeware for Population Genetic Analysis (POPGENE, Ver.1.31)
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Nongrum, I., Kumar, S., Kumaria, S. et al. Genetic variation and gene flow estimation of Nepenthes khasiana Hook. F- A threatened insectivorous plant of India as revealed by RAPD markers. J. Crop Sci. Biotechnol. 15, 101–105 (2012). https://doi.org/10.1007/s12892-011-0070-y
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DOI: https://doi.org/10.1007/s12892-011-0070-y