Abstract
The northern dragonhead Dracocephalum ruyschiana is a plant species experiencing a dramatic population decline that has led to the species being listed on Red Lists for species in many European countries. Here we used restriction-site associated DNA sequencing to isolate and characterize a panel of 96 novel SNP markers from 44 individuals encompassing most of the species range in Norway. The 96 SNPs were adapted for the Fluidigm platform and evaluated by screening another 24 northern dragonheads from a population in southern Norway. The panel of SNP markers developed here are expected to be useful for elucidating genetic diversity and population genetic structure in the northern dragonhead.
This is a preview of subscription content, log in via an institution to check access.
References
Catchen J, Hohenlohe PA, Bassham S, Amores A, Cresko WA (2013) Stacks: an analysis tool set for population genomics. Mol Ecol 22(11):3124–3140. https://doi.org/10.1111/mec.12354
Directorate for Nature Management (2010) Action plan for the northern dragonhead Dracocephalum ruyschiana and dragonhead sap beetle Meligethes norvegicus Trondheim. DN-report 2010-5
Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Res 10(3):564–567. https://doi.org/10.1111/j.1755-0998.2010.02847.x
Lid J (2005) Norsk flora. 7 edn. Det Norske Samlaget, Oslo
Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in excel: population genetic software for teaching and research: an update. Bioinformatics 28(19):2537–2539. https://doi.org/10.1093/bioinformatics/bts460
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43(1):223–225
Solstad H, Elven R, Mjelde M, Pedersen O, Alsos IG, Stabbetorp OE, Gaarder G (2015) Vascular plants: Pteridophyta, Pinophyta, Magnoliophyta. In: Henriksen S, Hilmo O (eds) Norwegian red list for species. Norwegian Biodiversity Information Centre, Trondheim
Acknowledgements
We are grateful to Marie K. Brandrud for field assistance and Line B. Eriksen for laboratory assistance. This study was conducted as part of the research projects “Management of biodiversity and ecosystem services in spatially structured landscapes” (208434/F40) and “Development and implementation of genetic methods” and financed by the Research Council of Norway and the Norwegian Institute for Nature Research. Permissions to sample leaves were provided by the Directorate for Nature Management (2012 2009/3206 NAK-NK-STB and 2013 2013/3149 NAK-NK-STB) and the Norwegian Environment Agency (2014/4159).
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Kleven, O., Endrestøl, A., Evju, M. et al. SNP discovery in the northern dragonhead Dracocephalum ruyschiana. Conservation Genet Resour 11, 431–435 (2019). https://doi.org/10.1007/s12686-018-1045-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-018-1045-9