Abstract
100 SNP and one Indel markers were developed for Khaya using a combination of restriction associated DNA sequencing and low coverage MiSeq genome sequencing. The marker set was successfully used to genotype a set of 1919 individuals, representing 5 Khaya species from 18 African countries, using MassARRAY®iPLEX™ genotyping.
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Acknowledgments
The German Federal Ministry of Food and Agriculture (BMEL), the International Tropical Timber Organization (ITTO), the Prince Albert II of Monaco Foundation, the CGIAR Research Program on Forests, Trees and Agroforestry (FTA), the Service Coopération et d’Action culturelle de l’Ambassade de France au Congo (SCAC), the Fondation international pour la Science, Centre de coopération International en Recherche Agronomique pour le Dévelopement (CIRAD) and Double Helix Tracking Technologies supported the work. MassARRAY®iPLEX™ screening was carried out at the facilities of the INRA Centre Bordeaux Aquitaine (France). We thank Vivian Kuhlenkamp and Susanne Bein for their excellent technical assistance.
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Pakull, B., Mader, M., Kersten, B. et al. Development of nuclear, chloroplast and mitochondrial SNP markers for Khaya sp.. Conservation Genet Resour 8, 283–297 (2016). https://doi.org/10.1007/s12686-016-0557-4
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DOI: https://doi.org/10.1007/s12686-016-0557-4