Abstract
182 SNP markers were developed for Ayous (Triplochiton scleroxylon K. Schum) by incorporating information from two next generation sequencing approaches (RADseq Floragenex and AFLPseq IonTorrent PGM) into a single genotyping panel for MassARRAY® iPLEX™. This set of markers was successfully used to genotype 753 individuals from 43 populations in five Tropical West and Central African Countries. These loci have an expected heterozygosity range of 0.007–0.501 and F ST from 0 to 0.306.
References
Akinnagbe A (2008) Genetic variation in Mansonia altissima A. Chev. and Triplochiton scleroxylon K. Schum under different regimes of human impact in Akure Forest Reserve, Nigeria. Dissertation, University of Göttingen
Baird NA, Etter PD, Atwood TS, Currey MC, Shiver AL, Lewis ZA, Selker EU, Cresko WA, Johnson EA (2008) Rapid SNP discovery and genetic mapping using sequenced RAD markers. PLoS ONE 3:e3376
Bosu PP, Krampah E (2005) Triplochiton scleroxylon K. Schum In: Louppe, D Oteng-Amoako AA, Brink M (eds) PROTA. (www.prota4u.org/search.asp). Accessed 12 Dec 2015
Degen B, Henry B (2015) Verifying timber in Africa. In: Carillo R (ed) ITTO tropical forest update 24(1):8–10
Dormontt EE, Boner M, Braun B, Breulmann G, Degen B, Espinoza E, Gardner S, Guillery P, Hermanson JC, Koch G (2015) Forensic timber identification: it’s time to integrate disciplines to combat illegal logging. Biol Conserv 191:790–798
Dumolin S, Demesure B, Petit R (1995) Inheritance of chloroplast and mitochondrial genomes in pedunculate oak investigated with an efficient PCR method. Theor Appl Genet 91:1253–1256
Hall JB, Bada S (1979) The distribution and ecology of Obeche (Triplochiton scleroxylon). J Ecol 67:543–564
Hardy OJ, Born C, Budde K, Daïnou K, Dauby G, Duminil J, Ewédjé E-EB, Gomez C, Heuertz M, Koffi GK (2013) Comparative phylogeography of African rain forest trees: a review of genetic signatures of vegetation history in the Guineo-Congolian region. CR Geosci 345:284–296
Igboanugo ABI, Iversen P (2004) Conservation and use of Triplochiton scleroxylon in moist West Africa. In: Iversen P, Sigaud P, France-Lanord M (eds) Forest Genetic Resources 31:50–52
ITTO (2015) Biennial review and assessment of the world timber situation 2013–2014. Appendix 3:125–187
Jardine D, Dormontt E, van Dijk K-J, Dixon R, Dunker B, Lowe A (2015) A set of 204 SNP and INDEL markers for Bigleaf maple (Acer macrophyllum Pursch). Conservation Genetics Resources 7:797–801
Meirmans PG, Van Tienderen PH (2004) GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms. Mol Ecol Notes 4:792–794
Orwa C, Mutua A, Kindt R, Jamnadass R, Simons A (2009) Agroforestree database: a tree reference and selection guide. Version 4.0. (www.worldagroforestry.org/af/treedb/). Accessed 12 Dec 2015
Rousset F (2008) Genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Resour 8:103–106
Slavov G, Nipper R, Robson P, Farrar K, Allison G, Bosch M, Clifton-Brown J, Donnison I, Jensen E (2014) Genome-wide association studies and prediction of 17 traits related to phenology, biomass and cell wall composition in the energy grass Miscanthus sinensis. New Phytol 201:1227–1239
Acknowledgments
The work was financially supported by the International Tropical Timber Organization (Project PD 620/11 Rev.1) and the Australian Research Council (Project LP120100648). The TIFG approach for loci screening was supported by funding from the Conseil Regional d’Aquitaine (Nos. 20030304002FA and 20040305003FA), European Union, FEDER (No. 2003227) and from Investissements d’avenir, Convention attributive d’aide (No. ANR-10-EQPX-16-01).
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Jardine, D.I., Blanc-Jolivet, C., Dixon, R.R.M. et al. Development of SNP markers for Ayous (Triplochiton scleroxylon K. Schum) an economically important tree species from tropical West and Central Africa. Conservation Genet Resour 8, 129–139 (2016). https://doi.org/10.1007/s12686-016-0529-8
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DOI: https://doi.org/10.1007/s12686-016-0529-8