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Species identification and molecular sexing from feces of Kashmir stag (Cervus elaphus hanglu)

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Abstract

We developed an easy and efficient molecular genetics technique where species identification and sexing of Kashmir stag (Cervus elaphus hanglu) was carried out simultaneously in a single PCR. The technique has assigned the sexes of known and unknown individuals without any ambiguity and has proven its applicability in identifying sexes of several other cervids including chital, sambar and hog deer.

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References

  • Arandjelovic M, Head J, Rabanal LI, Schubert G, Mettke E et al (2011) Non-invasive genetic monitoring of wild central chimpanzees. PLoS One 6(3):e14761. doi:10.1371/journal.pone.0014761

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Balakrishnan CN, Monfort SL, Gaur A, Singh L, Sorenson MD (2003) Phylogeography and conservation genetics of Eld’s deer (Cervus eldi). Mol Ecol 12:1–10

    Article  CAS  PubMed  Google Scholar 

  • Barbosa AM, Fernández-García JL, Carranza J (2009) A new marker for rapid sex identification of red deer (Cervus elaphus). Hystrix Ital J Mammal (n.s.) 20(2):169–172

    Google Scholar 

  • Bellemain E, Nawaz MA, Valentini A, Swenson JE, Taberlet P (2007) Genetic tracking of the brown bear in northern Pakistan and implications for conservation. Biol Conserv 2007(134):537–547

    Article  Google Scholar 

  • Chancellor RL, Langergraber K, Ramirez S, Rundus AS, Vigilant L (2012) Genetic sampling of unhabituated chimpanzees (Pan troglodytes schweinfurthii) in Gishwati forest reserve, an isolated forest fragment in Western Rwanda. Int J Primatol 33:479–488

    Article  Google Scholar 

  • Charoo SA, Sharma LK, Sathyakumar S (2010) Distribution and relative abundance of Hangul (Cervus elaphus hanglu) in Dachigam National Park. Span J Wildl Galemys 22 (n° especial): 171–184

  • Fain SR, LeMay JP (1995) Gender identification of humans and mammalian wildlife species from PCR amplified sex linked genes. Proc Am Acad Forensic Sci 1:34

    Google Scholar 

  • Huber S, Bruns U, Arnold W (2002) Sex determination of red deer using polymerase chain reaction of DNA from feces. Wildl Soc B 30:208–212

    Google Scholar 

  • Ludt CJW, Schroeder O, Rottmann O, Kuehn R (2004) Mitochondrial DNA phylogeography of red deer (Cervus elaphus). Mol Phylogenet Evol 31:1064–1083

    Article  CAS  PubMed  Google Scholar 

  • Mukesh, Sharma LK, Charoo SA, Sathyakumar S (2013a) An improved and reliable molecular sexing technique for Asiatic black bears Ursus thibetanus. Conserv Genet Resour 5:1079–1082

    Article  Google Scholar 

  • Mukesh, Sharma LK, Kumar VP, Charoo SA, Mohan N, Sathyakumar S, Goyal SP (2013b) Loss of genetic diversity and inbreeding in Kashmir red deer (Cervus elaphus hanglu) of Dachigam National Park, Jammu & Kashmir, India. BMC Res Notes 6:326

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Mukesh, Kumar VP, Sharma LK, Shukla M, Sathyakumar S (2015) Pragmatic perspective on conservation genetics and demographic history of the last surviving population of Kashmir red deer (Cervus elaphus hanglu) in India. PLoS One. doi:10.1371/journal.pone.0117069

    PubMed Central  PubMed  Google Scholar 

  • Paetkau D, Strobeck C (1994) Microsatellite analysis of genetic variation in black bear populations. Mol Ecol 3:489–495

  • Pfeiffer I, Brenig B (2005) X- and Y-chromosome specific variants of the amelogenin gene allow sex determination in sheep (Ovis aries) and European red deer (Cervus elaphus). BMC Genet 6:16

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Pitra C, Fickel J, Meijaard E, Groves CP (2004) Evolution of old world deer. Mol Phylogenet Evol 33:880–895

    Article  CAS  PubMed  Google Scholar 

  • Randi E, Mucci N, Claro-Hergueta F, Bonnet A, Douzery E (2001) A mitochondrial DNA control region phylogeny of the cervinae: speciation in Cervus and implications for conservation. Anim Conserv 4:1–11

    Article  Google Scholar 

  • Schaller GB (1969) Observation on Hangul or Kashmir stag (Cervus elaphus hanglu). J Bombay Nat Hist Soc 66(1):1–7

    Google Scholar 

  • Silva TL, Godinho R, Castro D, Abáigar T, Brito JC, Alves PC (2014) Genetic identification of endangered North African ungulates using noninvasive sampling. Mol Ecol Resour. doi:10.1111/1755-0998.12335

    Google Scholar 

  • Taberlet P, Bouvet J (1992) Bear conservation genetics. Nature 3(58):197

    Article  Google Scholar 

  • Thakur M, Rai ID, Mandhan RP, Sathyakumar S (2011) A panel of polymorphic microsatellite markers in Himalayan monal Lophophorus impejanus developed by cross-species amplification and their applicability in other Galliformes. Eur J Wildlife Res 57:983–989

    Article  Google Scholar 

  • Waits LP, Paetkau D (2005) Noninvasive genetic sampling tools for wildlife biologists: a review of applications and recommendations for accurate data collection. J Wildl Manag 69(4):1419–1433

    Article  Google Scholar 

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Acknowledgments

Authors are thankful to Director, Dean and Research Coordinator, Wildlife Institute of India, Dehradun for their encouragement and support. Our sincere thanks are due to the Department of Wildlife Protection of Jammu & Kashmir, particularly Mr. A.K. Singh, Chief Wildlife Warden and Mr. Rashid Y. Naqash, Wildlife Warden Central Division for providing necessary help and cooperation during the study.

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Mukesh, Sharma, L.K., Charoo, S.A. et al. Species identification and molecular sexing from feces of Kashmir stag (Cervus elaphus hanglu). Conservation Genet Resour 7, 677–680 (2015). https://doi.org/10.1007/s12686-015-0475-x

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  • DOI: https://doi.org/10.1007/s12686-015-0475-x

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