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Isolation, characterization and PCR multiplexing of 17 microsatellite loci in the pine processionary moth Thaumetopoea pityocampa (Lepidoptera, Notodontidae)

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Abstract

Seventeen polymorphic microsatellite markers were developed for the pine processionary moth, Thaumetopoea pityocampa and organized in three multiplex. The number of alleles ranged from 1 to 18 and observed heterozygosities from 0.068 to 0.892. Tests of cross-amplifications are also reported, and show that these loci can be used in divergent clades of the same species, and most of them for the sister species T. wilkinsoni. These markers will be useful to develop fine-scale population genetics study and adapt pest management strategies for this insect, which was proved to threaten relict pine populations in the Mediterranean Basin.

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Acknowledgments

This work was supported by the ANR-10-JCJC-1705-01 GenoPheno. Data used in this work were (partly) produced through molecular genetic analysis technical facilities of the Labex “Centre Méditerranéen de l’Environnement et de la Biodiversité”. We gratefully thank Jérôme Rousselet and Manuela Branco for collecting insects samples.

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Authors and Affiliations

  1. UMR1062 CBGP (INRA, CIRAD, IRD, Montpellier Supagro), INRA, 755 avenue du campus Agropolis, CS 30016, 34988, Montferrier-sur-lez cedex, France

    L. Sauné, F. Abella & C. Kerdelhué

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  1. L. Sauné
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  2. F. Abella
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  3. C. Kerdelhué
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Correspondence to L. Sauné.

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Sauné, L., Abella, F. & Kerdelhué, C. Isolation, characterization and PCR multiplexing of 17 microsatellite loci in the pine processionary moth Thaumetopoea pityocampa (Lepidoptera, Notodontidae). Conservation Genet Resour 7, 755–757 (2015). https://doi.org/10.1007/s12686-015-0453-3

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  • DOI: https://doi.org/10.1007/s12686-015-0453-3

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