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Isolation and characterization of fifteen microsatellite markers for Sichuan Torrent Frog Amolops mantzorum (Anura: Ranidae)

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Abstract

Sichuan torrent frog, Amolops mantzorum, is widely distributed in high mountain stream of southwestern China. The expansion of small hydropower dams and the 2008 Wenchuan earthquake cause its populations sharply decreasing. A set of fifteen polymorphic microsatellite markers were isolated and characterized from transcriptome–derived microsatellite DNA for A. mantzorum. The isolated loci were all polymorphic and the number of alleles per locus ranged from 3 to 18. The observed and expected heterozygosity ranged from 0.1212 to 0.9394 and from 0.1169 to 0.9086, respectively. One locus showed significant departure from Hardy–Weinberg equilibrium, and there was no evidence of linkage disequilibrium. These microsatellites loci will provide important genetic data for study and conservation strategies for A. mantzorum.

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Acknowledgments

We thank Siqi Yuan for assistance in laboratory. This study was funded by National Natural Science Foundation of China (31272282 and 31372181).

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Authors and Affiliations

  1. College of Life Science, China West Normal University, Nanchong, 637002, People’s Republic of China

    Wei Luo & Qiong Wang

  2. Department of Herpetology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, People’s Republic of China

    Wei Luo, Yun Xia & Xiaomao Zeng

Authors
  1. Wei Luo
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  2. Yun Xia
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  3. Qiong Wang
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  4. Xiaomao Zeng
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Corresponding authors

Correspondence to Qiong Wang or Xiaomao Zeng.

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Luo, W., Xia, Y., Wang, Q. et al. Isolation and characterization of fifteen microsatellite markers for Sichuan Torrent Frog Amolops mantzorum (Anura: Ranidae). Conservation Genet Resour 7, 489–491 (2015). https://doi.org/10.1007/s12686-014-0403-5

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  • DOI: https://doi.org/10.1007/s12686-014-0403-5

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