Abstract
Allis shad Alosa alosa and twaite shad Alosa fallax are two related anadromous European clupeid species which have undergone a sharp decline in the past decades. We describe the development of 13 microsatellite markers for both species, based on 454 pyrosequencing technology. The number of alleles per locus ranged from 2 to 11 in A. alosa and from 4 to 13 in A. fallax. Gene diversity ranged from 0.364 to 0.800 for A. alosa and 0.226 to 0.849 for A. fallax. These markers will help in conservation genetics studies such as assessing the extent of population decline, defining appropriate conservation units and monitoring reintroduction programs.
References
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Acknowledgments
We thank our colleagues from the CRESCO laboratory (MNHN Dinard) and I. Lebel and Y. Abdallah from Migrateurs Rhone Méditerrannée for their help in collecting the samples. This study received funding from the European Regional Development Fund (Transnational program Interreg IV, Atlantic Aquatic Resource Conservation Project) and the French Ministry of Ecology and Sustainable Development (project ‘Amphihalins, cohérence du réseau N2000 en mer’).
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Rougemont, Q., Besnard, AL., Baglinière, JL. et al. Characterization of thirteen new microsatellite markers for allis shad (Alosa alosa) and twaite shad (Alosa fallax). Conservation Genet Resour 7, 259–261 (2015). https://doi.org/10.1007/s12686-014-0352-z
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DOI: https://doi.org/10.1007/s12686-014-0352-z