Abstract
We report the development of eight nuclear microsatellite loci (SSR) for Parolinia ornata Webb (Brassicaceae), an endemic species from the island of Gran Canaria (Canary Islands) that belongs to the Canarian endemic genus Parolinia. Following evaluation of 36 individuals sampled in one population, all loci showed polymorphism, and displayed 33 alleles overall. The number of alleles per locus ranged from three to eight, with an average of 4.38. This set of SSR markers can be very useful in further studies addressing the genetic managing and conservation of this endemic species, and across-taxa amplification and genotyping tests confirmed their utility in the other six species of this Canarian endemic genus, three of which are critically endangered.
References
Dellaporta S, Wood J, Hicks J (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1(4):19–21. doi:10.1007/bf02712670
IUCN (2012) IUCN Red list of threatened species. Version 2012.1. http://www.iucnredlist.org. Accessed 13 Sept 2013
Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program cervus accommodates genotyping error increases success in paternity assignment. Mol Ecol 16(5):1099–1106. doi:10.1111/j.1365-294X.2007.03089.x
Lance SL, Light JE, Jones KL, Hagen C, Hafner JC (2010) Isolation and characterization of 17 polymorphic microsatellite loci in the kangaroo mouse, genus Microdipodops (Rodentia: Heteromyidae). Conser Genet Resour 2(1):139–141. doi:10.1007/s12686-010-9195-4
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86(3):248–249
Acknowledgments
This research was funded by the project ENCLAVES (MAC/3/C141). We thank the European Union’s “Programa de Cooperación Transnacional Madeira-Azores-Canarias” for support.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
González-Pérez, M.A., Caujapé-Castells, J. Development and characterization of nuclear microsatellite markers for Parolinia ornata Webb (Brassicaceae), and cross-species amplification in all species described in the Canarian endemic genus Parolinia . Conservation Genet Resour 6, 705–706 (2014). https://doi.org/10.1007/s12686-014-0192-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-014-0192-x