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A suite of multiplexed microsatellite loci for the ground beetle Abax parallelepipedus (Piller and Mitterpacher, 1783) (Coleoptera, Carabidae)

  • Microsatellite Letters
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Abstract

We report two sets of polymorphic, multiplexed microsatellite markers for the ground beetle Abax parallelepipedus. As the species is flightless, restricted to forests and affected by habitat fragmentation it can serve as a model species for landscape and conservation genetics. A complete set of 20 loci can be amplified in five PCR reactions and sequenced in two rounds, and a subset of 14 loci can be analyzed together in one PCR run and one sequencing round. In a scan of 3,432 individuals from across Germany using the 14 loci subset, we found between three and 14 alleles per locus. After accounting for two loci that are apparently sex-linked, no significant deviations from Hardy–Weinberg equilibrium were found. None of the loci showed evidence for the presence of null alleles. No overall linkage disequilibrium was detected. Some of the loci can also be used to study other Abax species.

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References

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Acknowledgments

The work was funded by the Deutsche Forschungsgemeinschaft (DFG) Priority Program 1374 “Infrastructure-Biodiversity-Exploratories”. Field work permits were issued by the relevant state environmental offices. Material from Slovenia was provided by Katharina Homburg and Al Vrezec.

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Authors and Affiliations

  1. Institute of Ecology, Leuphana University, Scharnhorststraße 1, 21335, Lüneburg, Germany

    Tamar Marcus & Thorsten Assmann

  2. Department of Community Ecology (BZF), Helmholtz Centre for Environmental Research UFZ, Theodor-Lieser-Strasse 4, 06120, Halle, Germany

    Walter Durka

  3. Zoological Institute and Museum, Biozentrum Grindel, University of Hamburg, Martin-Luther-King-Platz 3, 20146, Hamburg, Germany

    Claudia Drees

Authors
  1. Tamar Marcus
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  2. Thorsten Assmann
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  3. Walter Durka
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  4. Claudia Drees
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Correspondence to Tamar Marcus.

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Marcus, T., Assmann, T., Durka, W. et al. A suite of multiplexed microsatellite loci for the ground beetle Abax parallelepipedus (Piller and Mitterpacher, 1783) (Coleoptera, Carabidae). Conservation Genet Resour 5, 1151–1156 (2013). https://doi.org/10.1007/s12686-013-9985-6

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  • DOI: https://doi.org/10.1007/s12686-013-9985-6

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