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Development and characterization of four moderate multiplex microsatellite panels in crucian carp (Carassius auratus)

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Abstract

Sixteen microsatellite loci of Carassius auratus were successfully assigned into four multiplex panels in this study. These multiplex panels were characterized in 30 diploid C. auratus individuals from Songhua River. All loci were found to be polymorphic with the polymorphic information content ranging from 0.409 to 0.905. The number of alleles per locus varied from 3 to 16 and the observed heterozygosity (H O ) ranged from 0.269 to 0.897. Three of these loci significantly deviated from Hardy–Weinberg expectations and no pairs of loci showed significant linkage disequilibrium. These multiplex microsatellite panels will be used to determine population structure of C. auratus, and detect hybridization between closely related carps.

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Acknowledgments

We would like to thank Ms. Jing Wu and Ms. Ya-nan Wu for their technical assistance. This study was supported by Central-Level Non-profit Scientific Research Institutes Special Funds (No. HSY201304) and China Ministry of Agriculture “948” Program (No. 2011-G12).

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Authors and Affiliations

  1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, China

    Lei Cheng, Cui-Yun Lu, Chao Li & Xiao-Wen Sun

  2. The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing, 100141, China

    Yan Zhang

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  1. Lei Cheng
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  2. Yan Zhang
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  3. Cui-Yun Lu
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  4. Chao Li
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  5. Xiao-Wen Sun
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Correspondence to Xiao-Wen Sun.

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Cheng, L., Zhang, Y., Lu, CY. et al. Development and characterization of four moderate multiplex microsatellite panels in crucian carp (Carassius auratus). Conservation Genet Resour 5, 821–823 (2013). https://doi.org/10.1007/s12686-013-9917-5

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  • DOI: https://doi.org/10.1007/s12686-013-9917-5

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