Skip to main content
SpringerLink
Log in

Development of seventeen novel microsatellite markers of a wild and flowering bamboo Dendrocalamus membranaceus (Poaceae) and cross-amplification in Dendrocalamus genus

  • Microsatellite Letters
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

17 novel microsatellite markers were developed in Dendrocalamus membranaceus Munro, a declining and flowering wild bamboo in Lancang–Mekong River Valley, using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. The number of alleles per locus ranged from 2 to 13, with an average of 5.5 per allele. The observed and expected heterozygosities varied from 0.043 to 0.696 and from 0.479 to 0.926, respectively. Cross-amplifications were carried out for seven species of Dendrocalamus Nees, and resulted in a 41.2–100 % of successful amplification. These markers will be useful for detecting the gene flow in the flowering populations and studying its breeding system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Offerman J, Rychlik W (2003) Oligo primer analysis software. In: Krawetz S, Womble D (eds) Introduction to bioinformatics: a theoretical and practical approach. Humana Press, Totowa, pp 345–361

    Chapter  Google Scholar 

  • Raymond M, Rousset F (1995) Genepop (Version 4.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Yang HQ, An MY, Gu ZJ, Tian B (2012) Genetic diversity and differentiation of Dendrocalamus membranaceus (Poaceae: Bambusoideae), a declining bamboo species in Yunnan, China, as based on inter-simple sequence repeat (ISSR) analysis. Int J Mol Sci 13:4446–4457

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors thank Zhi-Rong Zhang for helping with laboratory work. This work was funded by projects of the National Natural Science Foundation of China (31270662, 31070593), Applied Basic Research Program of Yunnan Province, China (2010CD141) and Research Institute of Resources Insects (riricaf2012005M).

Author information

Authors and Affiliations

  1. Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming, 650224, Yunnan, China

    Yu-Ran Dong & Han-Qi Yang

Authors
  1. Yu-Ran Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Han-Qi Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Han-Qi Yang.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 33 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dong, YR., Yang, HQ. Development of seventeen novel microsatellite markers of a wild and flowering bamboo Dendrocalamus membranaceus (Poaceae) and cross-amplification in Dendrocalamus genus. Conservation Genet Resour 6, 201–203 (2014). https://doi.org/10.1007/s12686-013-0055-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-013-0055-x

Keywords

Search

Navigation