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Development of novel microsatellite markers for Cymodocea rotundata Ehrenberg (Cymodoceaceae), a pioneer seagrass species widely distributed in the Indo-Pacific

  • Microsatellite Letters
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Abstract

Genetic diversity and population genetic structure are key components of seagrass resilience and contribute to an understanding of its conservation and management. We isolated 29 polymorphic microsatellite (SSR) markers from a widely distributed pioneer seagrass, Cymodocea rotundata, by two methods; next generation sequencing and compound SSR marker isolation. Twenty-nine markers had 2–14 alleles per locus, and the observed and expected heterozygosity ranged from 0 to 0.688, and 0.113 to 0.770, respectively. These loci will facilitate investigation of the genetic diversity and population genetic connectivity and structure of C. rotundata.

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Acknowledgments

This study was supported by the Japanese Science and Technology Agency/Japanese International Cooperation Agency-Science and Technology Research Partnership for Sustainable Development (JST/JICA-SATREPS) for the integrated Coastal Ecosystem Conservation and Adaptive Management project under Local and Global Environmental Impacts in the Philippines (CECAM).

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Authors and Affiliations

  1. Department of Mechanical and Environmental Informatics, Graduate School of Information Science and Engineering, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro, Tokyo, 152-8552, Japan

    Dan M. Arriesgado & Kazuo Nadaoka

  2. Asian Natural Environmental Science Center, The University of Tokyo, 1-1-8 Midori-cho, Nishitokyo, Tokyo, 188-0002, Japan

    Dan M. Arriesgado, Yuichi Nakajima, Yu Matsuki & Chunlan Lian

  3. Harmful Algal Bloom Division, Toxic Phytoplankton Section, National Research Institute of Fisheries and Environment of Inland Sea, 2-17-5 Maruishi, Hatsukaichi, Hiroshima, 739-0452, Japan

    Satoshi Nagai, Motoshige Yasuike & Yoji Nakamura

  4. Marine Science Institute CS, University of the Philippines, Diliman, 1101, Quezon City, Philippines

    Miguel D. Fortes

  5. Institute of Fisheries Research and Development, Mindanao State University, 9023, Naawan, Misamis Oriental, Philippines

    Dan M. Arriesgado & Wilfredo H. Uy

  6. Division of Biological Sciences, College of Arts & Sciences, University of the Philippines Visayas, 5023, Miag-ao, Iloilo, Philippines

    Wilfredo L. Campos

  7. Akkeshi Marine Station, Field Science Center for Northern Biosphere, Hokkaido University, Aikappu, Akkeshi, Hokkaido, 088-1113, Japan

    Masahiro Nakaoka

Authors
  1. Dan M. Arriesgado
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  2. Yuichi Nakajima
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  3. Yu Matsuki
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  4. Chunlan Lian
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  5. Satoshi Nagai
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  6. Motoshige Yasuike
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  7. Yoji Nakamura
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  8. Miguel D. Fortes
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  9. Wilfredo H. Uy
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  10. Wilfredo L. Campos
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  11. Masahiro Nakaoka
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  12. Kazuo Nadaoka
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Corresponding author

Correspondence to Chunlan Lian.

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Arriesgado, D.M., Nakajima, Y., Matsuki, Y. et al. Development of novel microsatellite markers for Cymodocea rotundata Ehrenberg (Cymodoceaceae), a pioneer seagrass species widely distributed in the Indo-Pacific. Conservation Genet Resour 6, 135–138 (2014). https://doi.org/10.1007/s12686-013-0025-3

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  • DOI: https://doi.org/10.1007/s12686-013-0025-3

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