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Development of EST-derived microsatellite markers in the pearl oyster Pinctada martensii (Dunker) for genetic resource monitoring

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Abstract

Seventeen microsatellite markers were isolated from expressed sequence tags (ESTs) of Pinctada martensii for genetic resource monitoring. All loci amplified consistently in a wild Sanya Bay population and in a hatchery strain produced by intraspecific cross breeding. The number of alleles per locus ranged from 3 to 11 as characterized in 21 wild pearl oysters, and 4 to 8 in 21 hatchery individuals. The expected and observed heterozygosities ranged from 0.50 to 0.88, 0.10 to 0.86 in wild population, and 0.38 to 0.85, 0.10 to 0.91 in the hatchery, respectively. Most of those loci are in Hardy–Weinberg equilibrium (P > 0.05 after Bonferroni correction). Results revealed significant genetic differentiation between the wild and hatchery populations. These markers should be useful for population genetics studies and genetic resource monitoring in this species.

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Acknowledgments

This study was supported by the National Basic Research Program of China (973 Program 2010CB126405), the National Science Foundation of China (40866003 and 31060354) and Hainan Natural Science Foundation (#311024).

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Authors and Affiliations

  1. Key Laboratory of Tropical Biological Resources of Ministry of Education, Marine Biology Experiment Teaching Demonstration Center, Ocean College, Hainan University, 58 Renmin Road, Haikou, 570228, Hainan, China

    Ying Qiu, Xuzhen Huang, Hu Lu, Yaohua Shi, Aimin Wang & Yan Wang

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  1. Ying Qiu
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  2. Xuzhen Huang
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  3. Hu Lu
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  4. Yaohua Shi
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  5. Aimin Wang
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  6. Yan Wang
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Correspondence to Yan Wang.

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Qiu, Y., Huang, X., Lu, H. et al. Development of EST-derived microsatellite markers in the pearl oyster Pinctada martensii (Dunker) for genetic resource monitoring. Conservation Genet Resour 5, 401–403 (2013). https://doi.org/10.1007/s12686-012-9813-4

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  • DOI: https://doi.org/10.1007/s12686-012-9813-4

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