Abstract
14 Polymorphic microsatellite markers were developed in the Garra orientalis. These new markers were tested in 23 individuals collected from the Wanquan River in the Hainan Island. The number of alleles (NA), polymorphism information content, observed and expected heterozygosity per locus ranged from 8 to 25, 0.6788 to 0.9342, 0.5652 to 1.0000 and 0.7179 to 0.9585, respectively. Four loci are deviated from Hardy–Weinberg equilibrium due to the heterozygote deficiency. These markers can be used to study genetic diversity, population structure, conservation and rational utilization of this species.
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Acknowledgments
This research was supported by the National Natural Science Foundation of China (No. 30800099 and No. 31172066) and Shanghai Leading Academic Discipline Project (No. S30701).
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Li-Wei Su and Zhi-Zhi Liu contributed equally to this work.
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Su, LW., Liu, ZZ., Wang, CT. et al. Isolation and characterization of polymorphic microsatellite markers in the fish Garra orientalis (oriental sucking barb). Conservation Genet Resour 5, 231–233 (2013). https://doi.org/10.1007/s12686-012-9776-5
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DOI: https://doi.org/10.1007/s12686-012-9776-5