Abstract
We isolated and characterized eleven polymorphic tetranucleotide microsatellite loci in the spiny-bellied frog (Quasipaa boulengeri). The loci were screened in 62 individuals from two natural populations. All loci were variable, with the number of alleles ranging from 8 to 26. The average observed and expected heterozygosity per locus ranged from 0.500 to 0.947 and from 0.735 to 0.932, respectively. Two loci were found to be significant departure from Hardy–Weinberg equilibrium and no significant linkage disequilibrium was detected after sequential Bonferroni corrections. These polymorphic loci will be useful for conservation and population genetics studies of this threated species.
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Acknowledgments
We are grateful to Liyan Qing, Xiaoai Chang and Xiuyue Zhang for their help with experiments and data analysis. This project was supported by National Natural Science Foundation of China (NSFC-30870287, NSFC-30900134) and by Chinese Academy of Sciences (09C3011100, KSCX2-EW-J-22, KSCX2-YW-Z-0906).
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Xia, Y., Hu, L., Shan, X. et al. Isolation and characterization of eleven polymorphic tetranucleotide microsatellite loci for Quasipaa boulengeri (Anura: Dicroglossidae). Conservation Genet Resour 5, 5–7 (2013). https://doi.org/10.1007/s12686-012-9716-4
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DOI: https://doi.org/10.1007/s12686-012-9716-4