Abstract
Labeo fimbriatus, the fringe-lipped peninsula carp, is a commercially important fish species next to Indian major carps. We isolated and characterized polymorphic microsatellite markers to be used as a tool for delineation of genetic stock of this species. Partial genomic libraries enriched for CT and GT repeat motifs generated 103 positive clones out of which 16 loci were found with flanking regions enough for designing primers. Thirty-two individuals of L. fimbriatus collected from wild were used to characterize the polymorphism. All the 16 loci were polymorphic with allele numbers ranging from 3 to 9. The observed and expected heterozygosity ranged from 0.093 to 0.833 and from 0.146 to 0.843, respectively. Nine loci were in agreement with Hardy–Weinberg equilibrium. No significant pair wise linkage disequilibrium was found among the loci. These markers would be very useful for characterization of natural populations of this species.
This is a preview of subscription content, log in via an institution to check access.
References
Balloux F, Lugonae MN (2002) The estimation of population differentiation with microsatellite markers. Mol Ecol 11:155–165
Bloor PA, Barker FS, Watts PC, Noyes HA, Kemp SJ (2001). Microsatellite libraries enrichment. Protocol available at: http://www. Genomics. liv. ac. uk/animal/research/microsatellite
Das P, Barat A, Meher PK, Ray PP, Majumdar D (2005) Isolation and characterization of polymorphic microsatellites in Labeo rohita and their cross species amplification in related species. Mol Ecol Notes 5:231–233
Jena J, Das PC, Mitra G, Patro B, Mohanta D, Mishra B (2011) Evaluation of growth performance of Labeo fimbriatus (Bloch), L. gonius (Hamilton) and Puntius gonionotus (Bleeker) in polyculture with Labeo rohita (Hamilton) during fingerlings rearing at varied densities. Aq. Aquaculture 319:493–496
Kowtal GV (1994) Endangered, vulnerable and rare/food fishes of the east coast river system. Nature conservators, muzaffarnagar(India) 1:57–61
Lewis PO, Zaykin D (2001) Genetic data analysis: Computer program for analysis of allelic data, Ver 1.0. Free program distributed by the authors over the internet available at http://hydrodyction.eeb.uconn.edu/people/plewis/software.php
McConnell SKJ, Leamon J, Skibinski DOF, Mair GC (2001) Microsatellite markers from the Indian major carp species, Catla catla. Mol Ecol Notes 1:115–116
Molur S, Walker S (1998) Report of the workshop on conservation assessment and management plan for freshwater fishes of India, Zoo Outreach Organisation, Conservation Breeding Specialist Group, India Coimbatore, India. 156 p report
Patel A, Das P, Swain SK, Meher PK, Jayasankar P, Sarangi N (2009) Development of 21 new microsatellite markers in Labeo rohita (rohu). Anim Genet 40:253–254
Punia P, Gupta HS, Singh RK, Mahindra V, Lal KK, Chauhan VS, Lakra WS (2006) Polymorphic microsatellite markers isolated from partially enriched genomic library of Chitala chitala. Mol Ecol Notes 6:1263–1265
You F, Huo N, Gu Y, Luo M, Ma Y, Hane D, Lazo G, Dvorak J, Anderson O (2008) BatchPrimer3: a high throughput web application for PCR and sequencing primer design. BMC Bioinformatics 9:253
Acknowledgments
The work presented here was supported by Indian Council of Agricultural Research, New Delhi. We are thankful to Director, CIFA for providing laboratory facilities to work. We are also thankful to Dr. S. Ayyappan, Secretary DARE and Director General, ICAR for his support and encouragement. Help and support provided by Vijayawada and Bangalore centers of CIFA during samples collection are duly acknowledged.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Swain, S., Das, S.P., Bej, D. et al. Isolation and characterization of sixteen microsatellite loci for fringe-lipped carp, Labeo fimbriatus . Conservation Genet Resour 4, 913–915 (2012). https://doi.org/10.1007/s12686-012-9672-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-012-9672-z