Abstract
We identified 247 unique ruff (Philomachus pugnax) microsatellite sequences. Primer sets were designed from 102 selected loci and tested in 12–24 individuals from a captive population. Sequence homology was used to assign locations in the chicken (Gallus gallus) and/or zebra finch (Taeniopygia guttata) genome for the majority of these loci. Fifty-two loci were found to be polymorphic and 47 of these were typed in known families. Forty-six loci displayed Mendelian inheritance including Ppu058, which was confirmed to be Z-linked by the complete absence of any heterozygous females.
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Acknowledgments
This work was performed at the NERC Biomolecular Analysis Facility–Sheffield supported by the UK Natural Environmental Research Council. The captive ruff colony was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC; to DBL) and LLF was supported by an NSERC studentship.
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Farrell, L.L., Dawson, D.A., Horsburgh, G.J. et al. Isolation, characterization and predicted genome locations of ruff (Philomachus pugnax, AVES) microsatellite loci. Conservation Genet Resour 4, 763–771 (2012). https://doi.org/10.1007/s12686-012-9639-0
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DOI: https://doi.org/10.1007/s12686-012-9639-0