Abstract
Using the dual-suppression polymerase chain reaction technique, 12 novel polymorphic nuclear microsatellite DNA markers were isolated from the tropical seagrass Enhalus acoroides. The isolated markers provided the polymorphisms of 2–13 alleles per locus in 3 populations located in Japan, China, and the Philippines. The values of the expected heterozygosity fell in the following ranges: 0.000–0.693 (Japan), 0.000–0.623 (China), and 0.000–0.765 (Philippines). Compared to the Philippines population (1 monomorphic locus), a relatively higher proportion of the SSR loci did not show polymorphism in the marginal habitats of E. acoroides (5 loci in Japan; 5 loci in China). These markers may be useful for population and conservation genetics of E. acoroides.
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Acknowledgments
This research was jointly funded by the Japan Science and Technology Agency (JST) and the Japan International Cooperation Agency (JICA), Science and Technology Research Partnership for Sustainable Development (SATREPS), for the project Coastal Ecosystem Conservation and Adaptive Management under Local and Global Environmental Impacts in the Philippines (CECAM project: http://www.cecam-project.net/).
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Nakajima, Y., Matsuki, Y., Lian, C. et al. Development of novel microsatellite markers in a tropical seagrass, Enhalus acoroides . Conservation Genet Resour 4, 515–517 (2012). https://doi.org/10.1007/s12686-012-9614-9
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DOI: https://doi.org/10.1007/s12686-012-9614-9