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Isolation of 12 microsatellite markers for geelbeck (Atractoscion aequidens (Cuvier, 1860), Sciaenidae), an overexploited marine fish

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Abstract

Geelbeck (Atractoscion aequidens) represents a valuable fishery resource in the Benguela Current region in southwestern Africa. Due to overexploitation the species is considered depleted in South Africa, and little information is available for the rest of its distribution in the region. Genetic studies of the species are essential to inform management plans for sustainable harvesting and conservation. To facilitate such studies 12 polymorphic microsatellite DNA loci were isolated from an enriched genomic library. Genotyping of 41 individuals revealed an average of 17.75 alleles per locus (range 2–37), and observed/expected heterozygosity per locus of 0.024–0.951 and 0.024–0.962, respectively. These loci constitute the first microsatellite markers isolated for geelbeck and are applicable to multiple areas of research on this species.

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Acknowledgments

This work was funded by a PhD fellowship (SFRH/BD/36176/2007) awarded to R Henriques by the Portuguese FCT (Foundation for Science and Technology). The authors would like to thank Warren Potts for help with sample collection in Angola.

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Authors and Affiliations

  1. Centre for Ecology, Evolution and Behavior, School of Biological Sciences, Royal Holloway University of London, Bourne Building, Egham Hill, Egham, TW20 0EX, UK

    Romina Henriques, Niall J. McKeown & Paul W. Shaw

  2. Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Penglais, Aberystwyth, SY23 3DA, UK

    Paul W. Shaw

Authors
  1. Romina Henriques
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  2. Niall J. McKeown
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  3. Paul W. Shaw
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Correspondence to Paul W. Shaw.

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Henriques, R., McKeown, N.J. & Shaw, P.W. Isolation of 12 microsatellite markers for geelbeck (Atractoscion aequidens (Cuvier, 1860), Sciaenidae), an overexploited marine fish. Conservation Genet Resour 4, 85–87 (2012). https://doi.org/10.1007/s12686-011-9480-x

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  • DOI: https://doi.org/10.1007/s12686-011-9480-x

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