Skip to main content
Log in

Characterisation and predicted genome locations of Leach’s storm-petrel (Oceanodroma leucorhoa) microsatellite loci (Procellariidae, Aves)

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Forty-one microsatellite loci were found to be polymorphic in the Leach’s storm-petrel Oceanodroma leucorhoa when characterised in 24 unrelated individuals sampled from a population located at Gull Island, Newfoundland, Canada. Sequence homology was used to assign a predicted chromosome location for 39 of the polymorphic loci. Four polymorphic microsatellite loci were Z-linked based on the typing of known sex individuals and/or sequence homology. The set of 37 autosomal markers will be suitable for population and parentage studies of the Leach’s storm-petrel (combined first parent non-exclusion probability <0.0001).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

References

  • Armour JAL, Neumann R, Gobert S, Jeffreys AJ (1994) Isolation of human simple repeat loci by hybridization selection. Hum Mol Genet 3(4):599–605. doi:10.1093/hmg/3.4.599

    Article  PubMed  CAS  Google Scholar 

  • Dawson DA (2007) Genomic analysis of passerine birds using conserved microsatellite loci. Dissertation, University of Sheffield, Sheffield, UK

  • Dawson DA, Burke T, Hansson B, Pandhal J, Hale MC, Hinten GN, Slate J (2006) A predicted microsatellite map of the passerine genome based on chicken–passerine sequence similarity. Mol Ecol 15(5):1299–1320

    Article  PubMed  CAS  Google Scholar 

  • Dawson DA, Åkesson M, Burke T, Pemberton JM, Slate J, Hansson B (2007) Gene order and recombination rate in homologous chromosome regions of the chicken and a passerine bird. Mol Biol Evol 24(7):1537–1552. doi:10.1093/molbev/msm071

    Article  PubMed  CAS  Google Scholar 

  • Dawson DA, Horsburgh GJ, Kupper C, Stewart IRK, Ball AD, Durrant KL, Hansson B, Bacon I, Bird S, Klein A, Krupa AP, Lee JW, Martin-Galvez D, Simeoni M, Smith G, Spurgin LG, Burke T (2010) New methods to identify conserved microsatellite loci and develop primer sets of high cross-species utility—as demonstrated for birds. Mol Ecol Resour 10(3):475–494. doi:10.1111/j.1755-0998.2009.02775.x

    Article  PubMed  CAS  Google Scholar 

  • Fridolfsson AK, Ellegren H (1999) A simple and universal method for molecular sexing of non-ratite birds. J Avian Biol 30(1):116–121

    Article  Google Scholar 

  • Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. In: Molecular evolution: producing the biochemical data, Part B, vol 395, Methods in enzymology, Elsevier Academic Press Inc, San Diego, pp 202–222

  • Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program cervus accommodates genotyping error increases success in paternity assignment. Mol Ecol 16(5):1099–1106

    Article  PubMed  Google Scholar 

  • Kenta T, Gratten J, Haigh NS, Hinten GN, Slate J, Butlin RK, Burke T (2008) Multiplex SNP-SCALE: a cost-effective medium-throughput single nucleotide polymorphism genotyping method. Mol Ecol Resour 8(6):1230–1238. doi:10.1111/j.1755-0998.2008.02190.x

    Article  PubMed  CAS  Google Scholar 

  • Klein A, Horsburgh GJ, Kupper C, Major A, Lee PLM, Hoffmann G, Matics R, Dawson DA (2009) Microsatellite markers characterized in the barn owl (Tyto alba) and of high utility in other owls (Strigiformes: AVES). Mol Ecol Resour 9(6):1513–1519. doi:10.1111/j.1755-0998.2009.02715.x

    Google Scholar 

  • Rice WR (1989) Analyzing tables of statistical tests. Evolution 43(1):223–225

    Article  Google Scholar 

  • Rousset F (2008) GENEPOP ′007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8(1):103–106. doi:10.1111/j.1471-8286.2007.01931.x

    Article  PubMed  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz MS (ed) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJ, pp 365–386

    Google Scholar 

  • Sun Z, Gomez-Diaz E, Bailie A, Friesen V (2009) Isolation and characterization of microsatellite loci for storm-petrels. Mol Ecol Resour 9(3):913–915

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We thank the Canada Wildlife Services and Greg Robertson for permission and help collecting blood samples. Terry Burke provided helpful advice. This work was funded by the Natural Environment Research Council (NERC) and performed at the NERC Biomolecular Analysis Facility at Sheffield, UK.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Anthony W. J. Bicknell.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bicknell, A.W.J., Dawson, D.A., Horsburgh, G.J. et al. Characterisation and predicted genome locations of Leach’s storm-petrel (Oceanodroma leucorhoa) microsatellite loci (Procellariidae, Aves). Conservation Genet Resour 3, 711–716 (2011). https://doi.org/10.1007/s12686-011-9439-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-011-9439-y

Keywords

Navigation