Abstract
The Next Generation Sequencing (pyrosequencing) technique allows rapid, low-cost development of microsatellite markers. We have used this technology to develop 14 polymorphic loci for the endangered meadow viper (Vipera ursinii). Based on 37,000 reads, we developed primers for 66 microsatellite loci and found that 14 were polymorphic. The number of alleles per locus varies from 1 to 12 (for 30 individuals tested). At a cost of about 1/3 that of a normal microsatellite development, we were able to define enough microsatellite markers to conduct population genetic studies on a non-model species.
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Acknowledgments
The authors are grateful to Jean-Pierre Baron and Arnaud Lyet for providing the samples and thank Matthieu Raemy and Hans-Peter Rusterholz (University of Basel) for help in the laboratory. Polymorphism was tested through the molecular genetic analysis technical facilities of the IFR119 ‘‘Montpellier Environnement Biodiversité’’.
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Metzger, C., Ferchaud, AL., Geiser, C. et al. New polymorphic microsatellite markers of the endangered meadow viper (Vipera ursinii) identified by 454 high-throughput sequencing: when innovation meets conservation. Conservation Genet Resour 3, 589–592 (2011). https://doi.org/10.1007/s12686-011-9411-x
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DOI: https://doi.org/10.1007/s12686-011-9411-x