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Thirteen polymorphic microsatellite markers for the European green toad Bufo viridis viridis, a declining amphibian species

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Abstract

We report 13 new polymorphic microsatellite markers for the European green toad Bufo viridis viridis (B. viridis subgroup), a declining amphibian from Central, Southeastern and Eastern Europe. Diversity at these loci estimated for 19 individuals ranged from two to ten alleles. Most of these primers also cross-amplify in related West-Mediterranean green toad species (Bufo balearicus, B. siculus and B. boulengeri). These microsatellites will be useful for conservation genetics of threatened Bufo viridis viridis populations and evolutionary studies of green toad taxa in secondary contact to examine hybridization.

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Acknowledgments

We thank Lucio Bonato, Nicola Novarini, Stefano Mazzotti, and G. Francesco Ficetola for providing samples that served, in part, to choose pure B. viridis viridis populations examined in this paper. This study was in part supported by a grant from the “Fondation 450ème anniversaire” of the University of Lausanne (November 23, 2009 to M.St.).

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Authors and Affiliations

  1. Department of Ecology and Evolution, University of Lausanne, Biophore, 1015, Lausanne, Switzerland

    Christophe Dufresnes, Caroline Betto-Colliard, Nicolas Perrin & Matthias Stöck

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  1. Christophe Dufresnes
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  2. Caroline Betto-Colliard
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  3. Nicolas Perrin
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  4. Matthias Stöck
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Correspondence to Matthias Stöck.

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Dufresnes, C., Betto-Colliard, C., Perrin, N. et al. Thirteen polymorphic microsatellite markers for the European green toad Bufo viridis viridis, a declining amphibian species. Conservation Genet Resour 3, 311–313 (2011). https://doi.org/10.1007/s12686-010-9350-y

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  • DOI: https://doi.org/10.1007/s12686-010-9350-y

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