Abstract
White-lipped peccaries, Tayassu pecari, are neotropical ungulates whose populations have been declining in numerous locations within their geographical distribution. Here we describe 16 microsatellite loci isolated from T. pecari and their cross-amplification in collared peccaries, Pecari tajacu. In 30 individuals of T. pecari, a total of 32 alleles were found in ten polymorphic loci, ranging from 2 to 8 alleles per locus with a mean of 3.2. The expected and observed heterozygosity ranged from 0.143 to 0.802 and from 0 to 0.704, respectively. Two loci deviated from Hardy–Weinberg equilibrium. In P. tajacu, nine loci were polymorphic with a mean of 3.2 alleles per locus. These molecular markers will be useful to study the genetic status of peccary populations and, consequently, to help their conservation.
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Acknowledgments
We are grateful to Rio Negro Farm and Vale Verde Farm for logistical support and to veterinarian Tatiana P. Freitas, Ezidio Arruda, and Celso Vicente for valuable help in the field work. This project was funded by FAPESP (BIOTA 2007/03392-6), UNIDERP, FMB, IBC, Earthwatch Institute, WCS Brazil, CI, CNPq, and CAPES. MG and PMGJr receive a fellowship from CNPq.
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Vecchia, A.C.D., Biondo, C., Sanches, A. et al. Isolation and characterization of microsatellite loci for white-lipped peccaries (Tayassu pecari) and cross-amplification in collared peccaries (Pecari tajacu). Conservation Genet Resour 3, 151–154 (2011). https://doi.org/10.1007/s12686-010-9311-5
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DOI: https://doi.org/10.1007/s12686-010-9311-5