Abstract
Twenty six polymorphic single-locus microsatellite loci were developed in golden mahseer, Tor putitora. The number of observed alleles per locus ranged from 5 to 16. The observed and expected heterozygosities ranged from 0.2721 to 0.9830 and from 0.4631 to 0.9216, respectively. No significant linkage disequilibrium was detected among the loci. Eight loci showed significant deviation from Hardy–Weinberg equilibrium, but none of them had evidence for null alleles. Twenty-four primer pairs cross-amplified each in Tor khudree and Naziritor chelynoides and 25 each in T. tor and T. mosal mahanadicus. These microsatellite loci should be useful for genetic diversity studies and conservation of golden mahseer and its closely related species.
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Acknowledgements
The authors are grateful to Dr. S. Ayyappan, Director General, Indian Council of Agricultural Research for the guidance, support and encouragement; and Dr. K. K. Musammilu of NBFGR Cochin Unit for his help in data analysis.
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An erratum to this article can be found at http://dx.doi.org/10.1007/s12686-011-9444-1
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Singh, D.P., Lakra, W.S., Goswami, M. et al. Isolation and characterization of 26 polymorphic microsatellite loci in golden mahseer, Tor putitora (Teleostei, Cyprinidae) and their cross-species amplification in four related species. Conservation Genet Resour 3, 141–145 (2011). https://doi.org/10.1007/s12686-010-9309-z
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DOI: https://doi.org/10.1007/s12686-010-9309-z