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Isolation and characterization of ten polymorphic microsatellite markers for three cryptic Gammarus fossarum (Amphipoda) species

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Abstract

The ecologically important stream invertebrate Gammarus fossarum is a morphospecies that includes at least three genetically differentiated biological species. We developed ten microsatellite markers and tested them in a total of 208 individuals from all three known cryptic species (types A, B and C). All markers were polymorphic and successfully amplified in type A, nine in type B and five in type C. There were up to 11 alleles per marker and species.

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Acknowledgments

We thank Lara Pfister, Aria Minder and Tania Torossi for technical support. Data analyzed in this paper were generated in the Genetic Diversity Centre of ETH Zürich, Switzerland. This project was funded by the CCES (Competence Center Environment and Sustainability of the ETH Domain) BioChange project.

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Authors and Affiliations

  1. Eawag, Swiss Federal Institute of Aquatic Science and Technology, Überlandstrasse 133, 8600, Dübendorf, Switzerland

    A. M. Westram, J. Jokela & I. Keller

  2. ETH Zürich, Institute of Integrative Biology, Universitätstrasse 16, 8092, Zürich, Switzerland

    A. M. Westram & J. Jokela

Authors
  1. A. M. Westram
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  2. J. Jokela
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  3. I. Keller
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Correspondence to A. M. Westram.

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Westram, A.M., Jokela, J. & Keller, I. Isolation and characterization of ten polymorphic microsatellite markers for three cryptic Gammarus fossarum (Amphipoda) species. Conservation Genet Resour 2 (Suppl 1), 401–404 (2010). https://doi.org/10.1007/s12686-010-9287-1

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  • DOI: https://doi.org/10.1007/s12686-010-9287-1

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