Abstract
Cichla monoculus is an endemic species from Amazon basin and one of the main predators in the Amazon Rivers. It is widely commercialized as food, becoming one of the main items in Amazonian fisheries. Despite its economic importance, there are few genetic population studies in its natural environment. The present contribution describes 12 microsatellite loci used to analyze 27 individuals of C. monoculus. The number of alleles for each locus ranged from 2 to 13. The observed (H O ) and expected (H E ) heterozygosity values ranged from 0.45 to 0.94 and 0.45 to 0.90, respectively. Out of 12 polymorphic loci, nine did not deviate from Hardy–Weinberg Equilibrium after Bonferroni correction. A cross-amplification test with five different species suggests potential transferability of all analyzed loci.
References
Billotte N, Lagoda PJR, Risterucci AM, Baurens FC (1999) Microsatellite-enriched libraries: applied methodology for the development of SSR markers in tropical crops. Fruits 54:277–288
Cordeiro GM, Taylor GO, Henry RJ (2003) Characterization of microsatellite markers from sugarcane (Saccharum sp.), a highly polyploidy species. Plant Sci 155:161–168
Creste S, Tulmann Neto A, Figueira A (2001) Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining. Plant Mol Biol Rep 19:299–306
Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS (1991) “Touchdown” PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 19:4008
Farias IP, Vasconcelos WR, Nunes MS, Mota E, Hrbek T (2005) Padrões genético-populacionais de peixes da Várzea. In: Renno JF, García C, Duponchelle F, Nuñes J (eds) Biología de las poblaciones de peces de la Amazônia y piscicultura. IIAP/IRP, Iquito, pp 91–95
Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available from http://www.unil.ch/izea/softwares/fstat.html
Lewis P, Zaykin D (2002) Genetic Data Analysis (GDA): Computer Program for the Analysis of Allelic Data (Software), version 1.1 (d12). http://alleyn.eeb.uconn.edu/gda/
Petrere-Júnior M (1996) Fisheries in large tropical reservoirs in South America. Lakes Reserv Res Manag 2:111–133
Risterucci AM, Duval MF, Rohde W, Billote N (2005) Isolation and characterization of microsatellite loci from Psidium guajava L. Mol Ecol Notes 5:745–748
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual 2nd Edition. Cold Spring Harbor Laboratory, New York
Sampaio AMBM, Kubitza F, Cyrino JEP (2000) Relação energia: proteína na nutrição do tucunaré. Scientia Agrícola 57:213–219
Temnykh S, DeClerck G, Lukashova A, Lipovich L, Catinhour S, McCouch S (2001) Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): frequency, length variation, transposon associations, and genetic marker potential. Genome 11:1441–1452
Tessier C, David J, This P, Boursiquot JM, Charrier A (1999) Optimizations of the choice of molecular markers for varietal identification in Vitis viniferal. Theor Appl Genet 98:171–177
Thompson KW (1979) Cytotaxonomy of 41 species of Neotropical Cichlidae. Copeia 1979:679–691
Van Oosterhout C, Hutchinson WF, Wills DP, Shipley P (2004) Microchecker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Yeh FC, Rong-Cai Y, Boyle T (1998) POPGENE version 1.31. University of Alberta, Center for International Forestry Research, Edmonton
Acknowledgments
We thank Izeni Farias for providing other Cichlid species samples. Gliciane Silva, Jordânia Arraes, Daniel Fagundes, Gisele Climaco and Vivian Brandão contributed with the present work. Funding: CAPES (PROCAD), FAPEAM, CNPq and INCT ADAPTA.
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Lima, M.P., Campos, T., Sousa, A.C.B. et al. Isolation and characterization of microsatellite markers for Cichla monoculus (Agassiz, 1831), an important freshwater fish in the Amazon. Conservation Genet Resour 2, 215–218 (2010). https://doi.org/10.1007/s12686-010-9240-3
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DOI: https://doi.org/10.1007/s12686-010-9240-3