Abstract
We present 14 microsatellite loci that were isolated from little spotted kiwi (LSK, Apteryx owenii). All loci cross-amplify in all kiwi species currently recognized except for one locus in a single species. Little spotted kiwi exhibited lower variation at these loci (mean number of alleles, H E) than other kiwi species, despite the markers having been developed for polymorphism in LSK and a far greater number of LSK genotyped than kiwi of other species. Reliable cross-species amplification and polymorphism make these markers promising new tools for the management of New Zealand’s threatened kiwi.
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Acknowledgments
Funding was provided by the Bank of New Zealand Save the Kiwi Trust, Victoria University of Wellington, and the New Zealand Department of Conservation (DOC) Research and Development Group. A postdoctoral grant from the Allan Wilson Centre for Molecular Ecology and Evolution supported KMR during the writing of this manuscript. Kiwi tissue samples were provided by DOC. This work took place under permits from the New Zealand Environmental Risk Management Authority (GMD07075) and DOC (WE/325/EXP), and with the support of Waiorua Bay Trust, Kaitiaki o Kapiti Trust, Te Atiawa Manawhenua ki te Tau Ihu Trust, Te Runanga o Ati Awa ki Whakarongotai, and the Wellington Tenths Trust.
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Ramstad, K.M., Pfunder, M., Robertson, H.A. et al. Fourteen microsatellite loci cross-amplify in all five kiwi species (Apteryx spp.) and reveal extremely low genetic variation in little spotted kiwi (A. owenii). Conservation Genet Resour 2 (Suppl 1), 333–336 (2010). https://doi.org/10.1007/s12686-010-9233-2
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DOI: https://doi.org/10.1007/s12686-010-9233-2