Abstract
Copaifera langsdorffii is a Neotropical tree with wide distribution in the Brazilian Atlantic rain forest and savanna. Although eight microsatellite loci (SSR) were developed in 2000 and have been widely used since then, there is yet no information about their inheritance, linkage and linkage disequilibrium (LD). Through the analysis of 28 open-pollinated (OP) progenies, the SSR loci revealed Mendelian inheritance and independent assortment. Using these progenies, young and adult trees LD was mainly detected in OP progenies. Our results show clear evidence that the eight SSR loci can be used without restriction in genetic diversity, mating system and parentage analysis.
References
Ciampi AY, Brondani RPV, Grattapaglia D (2000) Desenvolvimento de marcadores microssatélites para Copaifera langsdorffii Desf. (copaíba) Leguminosae–Caesalpinoideae e otimização de sistemas fluorescentes de genotipagem multiloco. Boletim de Pesquisa da Embrapa Recursos Genéticos e Biotecnologia 16:1–40
Creste S, Tulmann-Neto A, Figueira A (2001) Detection of single sequence repeat polymorphism in denaturing polyacrylamide sequencing gels by silver staining. Plant Mol Biol Rep 19:299–306. doi:10.1007/BF02772828
Doyle JJ, Doyle JL (1987) A rapid DNA isolation method for small quantities of fresh tissues. Phytochem Bull Bot Soc Am 19:11–15
Gillet E, Hattemer HH (1989) Genetic analysis of isoenzyme phenotypes using single tree progenies. Heredity 63:135–141. doi:10.1038/hdy.1989.84
Goudet J (2002) FSTAT (Version 2.9.3.2): a computer program to calculate F-statistics. http://www.unil.ch/izea/softwares/fstat.html
Hedrick PW (2005) Genetics of populations, 3rd edn. Jones and Bartlett, Boston
Martins K, Santos JD, Gaiotto FA, Moreno MA, Kageyama PY (2008) Population genetic structure of Copaifera langsdorffii Desf. (Leguminosae—Caesalpinioideae) in forest fragments in “Pontal do Paranapanema”, SP, Brazil. Rev Brasil Bot 31:61-69. doi: 10.1590/S0100-84042008000100007
Pärtel M, Kalamees R, Reier Ü, Tuvi E-L, Roosaluste E, Vellak A, Zobel M (2005) Grouping and prioritization of vascular plant species for conservation: combining natural rarity and management need. Biol Cons 123:271–278. doi:10.1016/j.biocon.2004.11.014
Roberdge J-M, Angelstam P (2004) Usefulness of the umbrella species concept as a conservation tool. Cons Biol 18:76–85. doi:10.1111/j.1523-1739.2004.00450.x
R Development Core Team (2008) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL http://www.R-project.org
Tarazi R, Mantovani A, Reis MS (2009) Fine-scale spatial genetic structure and allozymic diversity in natural populations of Ocotea catharinensis Mez. (Lauraceae). Conserv Genet. doi: 10.1007/s10592-009-9939-4
Weir BS (1996) Genetic data analysis II: methods for discrete population genetic data, 2nd edn. Sinaver Associates, Sunderland
Acknowledgments
The authors thank Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for funding this project. RT is supported by a FAPESP PhD scholarship (grant no. 2006/04490-9) and AMS is supported by a National Counsel of Technological and Scientific Development (CNPq) research fellowship. Special thanks to Laboratório de Reprodução e Genética de Espécies Arbóreas (University of São Paulo) for laboratory help.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Tarazi, R., Sebbenn, A.M., Mollinari, M. et al. Mendelian inheritance, linkage and linkage disequilibrium in microsatellite loci of Copaifera langsdorffii Desf.. Conservation Genet Resour 2, 201–204 (2010). https://doi.org/10.1007/s12686-010-9230-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-010-9230-5