Abstract
The swimming crab, Portunus trituberculatus (Miers, 1876) is the most widely fished species of crab in the world. Ten polymorphic microsatellite markers from P. trituberculatus were isolated and characterized from a microsatellite-enriched genomic library. The analysis of variability was performed in 33 specimens of China (Tianjin). The number of alleles per locus ranged from 6 to 12 (mean, Na = 9). Observed and expected heterozygosities ranged from 0.6897 to 1.0000 and 0.7340–0.9045, respectively. The polymorphism information content (PIC) ranged from 0.6807 to 0.8755 with an average of 0.8189. No loci that deviated significantly from Hardy–Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium were found between pairs of loci. These microsatellite markers will be helpful for analyzing population structure and assisting to develop and monitor hatchery management guidelines for commercially important fishery species.
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Acknowledgments
The research was supported by Open-end Funds of Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology (2007HS003).
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Zhu, K.C., Wang, W.M., Yan, B.L. et al. Development of polymorphic microsatellite markers for the swimming crab, Portunus trituberculatus (Miers, 1876). Conservation Genet Resour 2 (Suppl 1), 117–119 (2010). https://doi.org/10.1007/s12686-010-9227-0
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DOI: https://doi.org/10.1007/s12686-010-9227-0