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Characterization of microsatellite loci in the straw-colored fruit bat, Eidolon helvum (Pteropodidae)

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Abstract

Twenty microsatellite loci developed for the straw-colored fruit bat, Eidolon helvum, are described. These markers were used in multiplex PCRs to amplify genomic DNA from 142 individuals sampled from nine populations across Africa. Nineteen loci were polymorphic, with a mean number of alleles per locus of 16.2 (3–46). Observed and expected heterozygosity values ranged from 0.01 to 0.90 and 0.03 to 0.97, respectively. These markers will be used in combination with mitochondrial DNA sequences to investigate gene flow and the genetic metapopulation structure of E. helvum.

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Acknowledgments

The authors thank David Hayman for his initial work in Ghana and for providing tissue samples. We greatly appreciate assistance from the Kasanka Trust, Zambian Wildlife Authority, National Research Council of Malawi, Museums of Malawi, Malawi Department of Animal Health, Tanzanian Commission for Science and Technology, Tanzanian Wildlife Research Institute, Ugandan National Council for Science and Technology, and Makerere University. Additional samples were kindly provided by Javier Juste, Ivan Kuzmin, Guy Gembu Tungaluna and the Californian Academy of Sciences. We thank Jesús Aguirre for advice and technical assistance, and Melissa Nollet for processing Zambian samples. This work was funded by the Cambridge Infectious Disease Consortium, Charles Slater Trust, Jowett Fund and the ZSL Daisy Balogh Fund.

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Authors and Affiliations

  1. Department of Veterinary Medicine, University of Cambridge, Cambridge, UK

    Alison J. Peel, James L. N. Wood & David R. Sargan

  2. Institute of Zoology, Zoological Society of London, London, UK

    Alison J. Peel & Andrew A. Cunningham

  3. School of Biological and Chemical Sciences, Queen Mary, University of London, London, UK

    Stephen J. Rossiter

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  1. Alison J. Peel
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  2. Stephen J. Rossiter
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  3. James L. N. Wood
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  4. Andrew A. Cunningham
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  5. David R. Sargan
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Correspondence to Alison J. Peel.

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Peel, A.J., Rossiter, S.J., Wood, J.L.N. et al. Characterization of microsatellite loci in the straw-colored fruit bat, Eidolon helvum (Pteropodidae). Conservation Genet Resour 2 (Suppl 1), 279–282 (2010). https://doi.org/10.1007/s12686-010-9190-9

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  • DOI: https://doi.org/10.1007/s12686-010-9190-9

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