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Isolation and characterization of microsatellite loci in the endangered lizard Gallotia bravoana and cross-species amplification in other Canarian Gallotia

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Abstract

The Gallotia bravoana lizard, is an endangered species endemic to the Canary Island, entirely restricted to a very small area (~1 Ha) in La Gomera. Here we report the isolation and characterization of fifteen microsatellite loci following an enrichment protocol. Although five loci were monomorphic, an average of 2.1 alleles per locus and an average observed heterozygosity of 0.423 were found (n = 33) for the remaining loci. The loci were tested for their ability to cross amplify in all Gallotia species. These microsatellites will be used to manage a captive breeding programme for this endangered species.

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Acknowledgments

We would like to thank Monik Almeida, Yessi Lara and Almudena Ramos for laboratory assistance. The European Commission project LIFE06/NAT/E/000199 and the Cabildo de La Gomera co-financed this study. We also express our thanks to Mariano Hernández Ferrer for providing some samples and Susan Cranfield for language correction.

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Authors and Affiliations

  1. Departamento de Genética, Facultad de Medicina, Universidad de Las Palmas de Gran Canaria, 35080, Las Palmas, Gran Canaria, Spain

    N. M. Suárez, E. Betancor & J. Pestano

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  1. N. M. Suárez
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  2. E. Betancor
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  3. J. Pestano
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Correspondence to J. Pestano.

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Suárez, N.M., Betancor, E. & Pestano, J. Isolation and characterization of microsatellite loci in the endangered lizard Gallotia bravoana and cross-species amplification in other Canarian Gallotia . Conservation Genet Resour 2 (Suppl 1), 265–268 (2010). https://doi.org/10.1007/s12686-010-9183-8

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  • DOI: https://doi.org/10.1007/s12686-010-9183-8

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