Abstract
The Australian freshwater catfish (Tandanus tandanus) has suffered a decline in abundance and distribution, and stocking of wild populations with hatchery-bred fish has been suggested to assist with population recovery. Here we describe the isolation and characterisation of eight microsatellite markers that may be used to assess population structure of T. tandanus in the wild to inform future stocking programs of any major genetic boundaries between populations. We tested the variability of the loci in 28–29 individuals from three populations of T. tandanus, as well in 24 individuals from a population representing an undescribed species. Expected heterozygosity for these loci ranged from 0.034 to 0.920 across the four populations. All loci successfully amplified in the three T. tandanus populations, while in the undescribed species one locus failed to amplify and three loci were monomorphic.
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References
Beheregaray LB, Möller LM, Schwartz TS, Chao NL, Caccone G (2004) Microsatellite markers for the cardinal tetra Paracheirodon axelrodi, a commercially important fish from central Amazonia. Mol Ecol Notes 4:330–332
Clunie P, Koehn J (2001a) Freshwater catfish: a resource document Arthur Rylah Institute for Environmental Research. Department of Natural Resources and Environment, Heidelberg
Clunie P, Koehn J (2001b) Freshwater catfish: a recovery plan Arthur Rylah Institute for Environmental Research. Department of Natural Resources and Environment, Heidelberg
Fischer D, Bachmann K (1998) Microsatellite enrichment in organisms with large genomes (Allium cepa L.). BioTechniques 24:796–802
Harris JH, Battaglene SC (1990) The introduction and translocation of native freshwater fishes in south-eastern Australia. In: Pollard DA (ed) Australian Society of fish biology workshop. Proceedings No. 8. Magnetic Island, Townsville, pp 136–142
Jerry DR (2005) Electrophoretic evidence for the presence of Tandanus tandanus (Pisces: Plotosidae) immediately north and south of the Hunter River, New South Wales. Proc Linn Soc NSW 126:121–124
Jerry DR (2008) Phylogeography of the freshwater catfish Tandanus tandanus (Plotosidae): a model species to understand evolution of the eastern Australian fish fauna. Mar Freshwat Res 59:351–360
Jerry DR, Woodland DJ (1997) Electrophoretic evidence for the presence of the undescribed ‘Bellinger’ catfish (Tandanus sp.) (Teleostei: Plotosidae) in four New South Wales mid-northern coastal rivers. Mar Freshwat Res 48:235–240
Musyl MK, Keenan CP (1996) Evidence for cryptic speciation in Australian freshwater eel-tailed catfish, Tandanus tandanus (Teleostei: Plotosidae). Copeia 3:526–534
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Rice WF (1989) Analyzing tables of statistical tests. Evolution 43:223–225
Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual, 2nd edn. Cold Harbour Spring Laboratory, New York
Acknowledgments
The authors would like to thank the Recreational Freshwater Fishing Trust for providing financial assistance. We would also like to thank Cameron McGreggor, Max Graham, Gavin Butler, Bradley Mackay, Peter McLean, Jamie Hutchison and Tim McGarry for assistance with collecting samples. This research was conducted under the New South Wales ACEC permit no 05/06.
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Rourke, M.L., Teske, P.R., Attard, C.R.M. et al. Isolation and characterisation of microsatellite loci in the Australian freshwater catfish (Tandanus tandanus). Conservation Genet Resour 2 (Suppl 1), 245–248 (2010). https://doi.org/10.1007/s12686-009-9161-1
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DOI: https://doi.org/10.1007/s12686-009-9161-1