Abstract
We describe the first eleven microsatellite loci for hawkmoths in Hyles tithymali from the Canary Islands. They are polymorphic with 3–13 alleles per locus, an expected heterozygosity between 0.18 and 0.86, and an observed heterozygosity between 0.11 and 0.68. As typical for Lepidoptera, the yield of new loci was low due to the presence of microsatellite gene families, mildly repetitive and variable flanking regions. These first microsatellites provide informative results in population studies of Hyles tithymali, a thermophilic species hybridising with Hyles euphorbiae in the Mediterranean.
This is a preview of subscription content, log in via an institution to check access.
References
Alba MM, Laskowski RA, Hancock JM (2002) Detecting cryptically simple protein sequences using the SIMPLE algorithm. Bioinformatics 18:672–678
Guillot G, Santos F, Estoup A (2008) Analysing georeferenced population genetics data with Geneland: a new algorithm to deal with null alleles and a friendly graphical user interface. Bioinformatics 24:1406–1407
Hundsdoerfer AK, Wink M (2006) Incongruence of morphology and genetic markers in Hyles tithymali (Lepidoptera: Sphingidae) from the Canary Islands. J Zool Syst Evol Res 44:316–322
Hundsdoerfer AK, Kitching IJ, Wink M (2005) The phylogeny of the Hyles-euphorbiae-complex (Lepidoptera: Sphingidae): molecular evidence from sequence data and ISSR-PCR fingerprints. Org Div Evol 5:173–198
Hundsdoerfer AK, Rubinoff D, Attié M, Wink M, Kitching IJ (2009) A revised molecular phylogeny of the globally distributed hawkmoth genus Hyles (Lepidoptera: Sphingidae), based on mitochondrial and nuclear DNA sequences. Mol Phyl Evol 52:852–865
Kuulasmaa T, Javed AA, Muthusamy A (2004) Oligo Explorer 1.2. Genelink, Hawthorne, New York
Meglécz E, Solignac M (1998) Microsatellite loci for Parnassius mnemosyne (Lepidoptera). Hereditas 128:179–180
Meglécz E, Anderson SJ, Bourguet D et al (2004) High similarity between flanking regions of different microsatellites detected within each of two species of Lepidoptera: Parnassius apollo and Euphydryas aurinia. Mol Ecol 13:1693–1700
Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Schneider S, Roessli D, Excoffier L (2000) Arlequin: a software for population genetic data analysis. Ver. 2.000. Genetics and Biometry Lab, Department of Anthropology, University of Geneva
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16
Zhang DX (2004) Lepidopteran microsatellite DNA: redundant but promising. Trends Ecol Evol 19:507–509
Acknowledgments
We thank Christian Kehlmaier, Anke Müller and Ina Lehmann (Dresden) for laboratory work and Hilke Schröder (Grosshansdorf) for advice and primer design. Funding by the Deutsche Forschungsgemeinschaft (DFG; HU 1561/1-1 & 1-2) is gratefully acknowledged. Field work was financed by Michael Wink (Heidelberg). Thanks go to Canary islands government departments for collection licences.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hundsdoerfer, A.K., Sanetra, M., Corbeil, D. et al. Eleven hawkmoth microsatellite loci of Canary Island Hyles tithymali (Lepidoptera). Conservation Genet Resour 2 (Suppl 1), 241–244 (2010). https://doi.org/10.1007/s12686-009-9160-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-009-9160-2