Abstract
We describe the first panel of nuclear simple sequence repeats (SSRs) loci for Brachiaria humidicola (Rendle) Schweick., a warmseason grass with facultative apomixis, variation in ploidy levels (6X–9X), and important forage grass species in the Tropics. Of 38 pairs of primers obtained by using an enriched-library methodology, 27 revealed polymorphism in 58 accessions of the Germplasm Collection of B. humidicola held at Embrapa Beef Cattle, Brazil. Eleven loci amplified in B. dictyoneura, a closely related species with unclear taxonomic boundaries with B. humidicola. Transferability to other three Brachiaria species was also evaluated. The developed microsatellites are potentially useful for genetic studies of B. humidicola, as well as phylogenetic evaluations, conservation and breeding applications.
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References
Billotte N, Lagoda PJR, Risterucci AM, Baurens FC (1999) Microsatellite-enriched libraries: applied methodology for the development of SSR markers in tropical crops. Fruits 54:277–288
Catasús Guerra L (2001) De Poaceis Cubensibus Notulae, III (In: Floram Novam Cubensem Stvdia Intendentia). Fontqueria 55(4):15–17
Cordeiro GM, Pan YB, Henry RJ (2003) Sugarcane microsatellites for the assessment of genetic diversity in sugarcane germplasm. Plant Sci 165(1):181–189
Creste S, Tulmann Neto A, Figueira A (2001) Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining. Plant Mol Biol Rep 19:299–306
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Gonzalez AMT, Morton CM (2005) Molecular and morphological phylogenetic analysis of Brachiaria and Urochloa (Poaceae). Mol Phylogenet Evol 37(1):36–44
Grisebach A (1853) Gramineae. In: Ledebour CF (ed) Flora Rossica, vol 4. Sumptibus Librariae E. Schweizerbart, Stuttgart, pp 324–484
Jungmann L, Sousa ACB, Paiva J, Francisco PM, Vigna BBZ, Valle CB do, Zucchi MI, Souza AP (2009) Isolation and characterization of microsatellite markers for Brachiaria brizantha (Hochst. ex A. Rich.) Stap Conserv Genet. doi:10.1007/s10592-009-9839-7
Morrone O, Zuloaga FO (1992) Revisión de las Especies Sudamericanas Nativas e Introducidas de los Géneros Brachiaria y Urochloa (Poaceae: Panicoideae: Paniceae). Darwiniana 31:43–109
Renvoize SA, Clayton WD, Kabuye CHS (1996) Morphology, taxonomy, and natural distribution of Brachiaria (Trin.) Griseb. In: Miles JW, Maass BL, Valle CB, Valle do CB (eds) Brachiaria: biology, agronomy and improvement. Embrapa/CIAT, Cali, pp 1–15
Veldkamp JF (1996) Brachiaria, Urochloa (Gramineae—Paniceae) in Malesia. Blumea 41(2):413–437
Zuloaga FO, Morrone O (2003) Urochloa. In: Catalogue of New World grasses (Paleae): III. Subfamilies Panicoideae, Aristidoideae, Arundinoideae, and Danthonioideae, vol 46. Contributions from the United States National Herbarium, Washington, pp 629–634
Acknowledgements
The authors would like to thank Felipe Rodrigues da Silva for assisting with bioinformatics. This work was supported by grants from Brazilian Agricultural Research Corporation (EMBRAPA), Brazilian National Council for Scientific and Technological Development (CNPq), State of São Paulo Research Foundation (FAPESP) and Foundation for Science and Technological Development of the State of Mato Grosso do Sul (FUNDECT). The authors are also grateful to FUNDECT for graduate fellowship to Jungmann, L., FAPESP for undergraduate fellowships to Paiva, J. and Vigna, B.B.Z. and graduate fellowships to Sousa, A. C. B. and Laborda, P, R. and, to CNPq for a research fellowship awarded to Souza, A. P.
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Jungmann, L., Vigna, B.B.Z., Paiva, J. et al. Development of microsatellite markers for Brachiaria humidicola (Rendle) Schweick. Conservation Genet Resour 1, 475–479 (2009). https://doi.org/10.1007/s12686-009-9111-y
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DOI: https://doi.org/10.1007/s12686-009-9111-y