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Isolation and characterization of 11 polymorphic microsatellite loci for the Saurogobio dabryi (Teleostei: Cyprinidae)

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Abstract

Eleven polymorphic microsatellite loci for the Saurogobio dabryi were isolated from three repeat-enriched genomic libraries. Characterization of these loci were developed in 24 individuals with the number of alleles ranged from 2 to 14. Observed heterozygosity ranged from 0.17 to 1.00 and expected heterozygosity was from 0.16 to 0.93. Three loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction, and all pairwise tests for linkage disequilibrium among loci were nonsignificant. These novel loci will be used to investigate the population genetics and geographic differentiation in Saurogobio dabryi.

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Acknowledgements

We are grateful to Zeng Yu for sample collection. Funding support came from the National Natural Science Foundation of China (NSFC 40432003) the Cypriniformes Tree of Life (CToL) project funded by the U.S. National Science Foundation (NSFEF-0431326), and the key project of Chinese Academy of Sciences (KZCXI-YW-08-01).

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Authors and Affiliations

  1. Key Lab of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, Peoples Republic of China

    Dan Yu, Baoqing Ding & Huanzhang Liu

  2. Graduate School of the Chinese Academy of Sciences, 100039, Beijing, Peoples Republic of China

    Dan Yu

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  1. Dan Yu
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  2. Baoqing Ding
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  3. Huanzhang Liu
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Correspondence to Huanzhang Liu.

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Yu, D., Ding, B. & Liu, H. Isolation and characterization of 11 polymorphic microsatellite loci for the Saurogobio dabryi (Teleostei: Cyprinidae). Conservation Genet Resour 1, 471 (2009). https://doi.org/10.1007/s12686-009-9109-5

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  • DOI: https://doi.org/10.1007/s12686-009-9109-5

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