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Isolation and characterization of microsatellite loci for the cavehopper Palmorchestia hypogaea (Amphipoda: Talitridae)

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Abstract

Eight polymorphic microsatellite loci for the subterraneous microphtalmous Palmorchestia hypogaea (Amphipoda: Talitridae) were isolated from a genomic library enriched for AG, AC and AAC repetitive elements. The number of alleles ranged from 2 to 8 per locus with the observed heterozygosity ranging from 0.00 to 0.94 in the two studied populations. These variable markers will be useful in evaluating gene flow and population structure within the subterranean populations and should provide valuable information about the importance of dispersal through the underground in the dynamic of cave biotas.

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Acknowledgements

We thank Steven J. Cooper and Matthew Hayden (University of Adelaide) for providing training and technology. The Cabildo of La Palma is acknowledged for granting permission and accommodation during field trips. This study was funded by Ministerio de Educación y Ciencia, Spain, under the project CGL2006-01365.

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Authors and Affiliations

  1. Departament de Biologia, Universitat de les Illes Balears, Palma de Mallorca, Spain

    Carlos Villacorta & Carlos Juan

  2. Centro de Ciências do Mar do Algarve, Faro, Portugal

    Fernando Cánovas

  3. Departamento de Biología Animal, Universidad de La Laguna, La Laguna, Spain

    Pedro Oromí

Authors
  1. Carlos Villacorta
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  2. Fernando Cánovas
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  3. Pedro Oromí
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  4. Carlos Juan
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Correspondence to Carlos Villacorta.

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Villacorta, C., Cánovas, F., Oromí, P. et al. Isolation and characterization of microsatellite loci for the cavehopper Palmorchestia hypogaea (Amphipoda: Talitridae). Conservation Genet Resour 1, 401–404 (2009). https://doi.org/10.1007/s12686-009-9093-9

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  • DOI: https://doi.org/10.1007/s12686-009-9093-9

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