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Microsatellite markers in tropical legume (Centrosema pubescens Benth): development, characterization, and cross-species amplification in Centrosema sp

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Abstract

Centrosema pubescens Benth is a forage legume widespread in tropical America. Twenty-six polymorphic microsatellite markers were isolated and characterized in 15 genotypes of C. pubescens from the Cerrados Research Center Germplasm Bank of the Brazilian Agricultural Research Corporation (Embrapa). The number of alleles observed for each locus ranged from 2 to 5, with an average of 3 alleles per locus. The polymorphism information content (PIC) varied between 0.39 and 0.86 (average 0.57) and the discriminating power (D) ranged from 0.45 to 0.98 (average 0.68). The observed heterozygosity (H o) and the expected heterozygosity (H e) were 0.01–0.81 and 0.10–0.86, respectively. A cross-amplification test in 11 Centrosema species suggested potential transferability of these microsatellites. The data indicated that the polymorphic microsatellite markers developed in this work should be useful for assessing genetic diversity in further breeding programs and germplasm conservation.

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Acknowledgments

The authors would like to thank Fundação de Amparo à Pesquisa de São Paulo (FAPESP), for the financial support (Project Number 05/51010) and a graduate scholarship to Sousa, ACB; the Conselho Nacional de Pesquisa (CNPq) for graduate, undergraduate and research fellowships to Boaventura, LR, Sforça, DA, Carvalho, MA, Zucchi, MI and Souza, AP.

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Authors and Affiliations

  1. Centro de Biologia Molecular e Engenharia Genética, Departamento de Genética e Evolução, Universidade Estadual de Campinas, Barão Geraldo, CP 6010, Campinas, SP, CEP 13083-970, Brazil

    A. C. B. Sousa, L. R. Boaventura, D. A. Sforça, T. Campos & A. P. Souza

  2. Brazilian Agricultural Research Corporation (Embrapa Cerrados), BR 020, Km 18, Planaltina, DF, CEP 73310-970, Brazil

    M. A. Carvalho

  3. Brazilian Agricultural Research Corporation (Embrapa Gado de Corte), BR 262, Km 4, Campo Grande, MS, CEP 79002-970, Brazil

    L. Jungmann & L. Jank

  4. Instituto Agronômico de Campinas (IAC), Av. Barão de Itapura n 1481, CP 28, Campinas, SP, CEP 13012-970, Brazil

    M. I. Zucchi

  5. Instituto de Biologia, Departamento de Biologia Vegetal, Universidade Estadual de Campinas, Barão Geraldo, CP 6109, Campinas, SP, CEP 13083-970, Brazil

    A. P. Souza

Authors
  1. A. C. B. Sousa
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  2. M. A. Carvalho
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  3. L. R. Boaventura
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  4. D. A. Sforça
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  5. T. Campos
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  6. L. Jungmann
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  7. M. I. Zucchi
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  8. L. Jank
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  9. A. P. Souza
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Corresponding author

Correspondence to A. P. Souza.

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Sousa, A.C.B., Carvalho, M.A., Boaventura, L.R. et al. Microsatellite markers in tropical legume (Centrosema pubescens Benth): development, characterization, and cross-species amplification in Centrosema sp. Conservation Genet Resour 1, 347–352 (2009). https://doi.org/10.1007/s12686-009-9080-1

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  • DOI: https://doi.org/10.1007/s12686-009-9080-1

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