Abstract
We isolated and characterized 11 polymorphic microsatellite loci for Anolis distichus, a West Indian lizard endemic to Hispaniola and the Bahamas. The number of alleles per locus ranged from 10 to 34, with levels of observed heterozygosity ranging from 0.10 to 1.00. Four loci significantly deviated from Hardy–Weinberg equilibrium for most of the populations sampled, and seven pairs of loci demonstrated linkage disequilibrium for 1–2 populations. These markers provide a valuable set of tools for fine-scale population genetics studies of A. distichus, a species that exhibits striking geographic variation in dewlap coloration and pattern.
This is a preview of subscription content, log in via an institution to check access.
References
Case SM, Williams EE (1984) Study of a contact zone in the Anolis distichus complex in the central Dominican Republic. Herpetologica 40:118–137
Castelo AT, Martins W, Gao GR (2002) TROLL—tandem repeat occurrence locator. Bioinformatics 18:634–636
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Molecular Evolution: Producing the Biochemical Data, Part B, vol 395, pp 202–222
Greenberg B, Noble GK (1944) Social behavior of the American chameleon, Anolis carolinenesis Voight. Physiol Zool 17:392–439
Jenssen TA (1977) Evolution of anoline lizard display behavior. Am Zool 17:203–215
Losos JB (1985) An experimental demonstration of the species-recognition role of Anolis dewlap color. Copeia 1985:905–910
Rampling LR, Harker N, Shariflou MR, Morell MK (2001) Detection and analysis systems for microsatellite markers in wheat. Aust J Agric Res 52:1131–1141
Rand AS, Williams EE (1970) An estimation of redundancy and information content of anole dewlaps. Am Nat 104:99–103
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Schable NA, Fischer RU, Glenn TC (2002) Tetranucleotide microsatellite DNA loci from the dollar sunfish (Lepomis marginatus). Mol Ecol Notes 2:509–511
Schwartz A (1968) Geographic variation in Anolis distichus Cope (Lacertilia, Iguanidae) in the Bahama Islands and Hispaniola. Bull Mus Comp Zool 137:255–309
Sigmund WR (1983) Female preference for Anolis carolinensis males as a function of dewlap color and background coloration. J Herpetol 17:137–143
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
West-Eberhard MJ (1983) Sexual selection, social competition, and speciation. Q Rev Biol 58:155–183
Williams EE (1975) Anolis marcanoi new species: sibling to Anolis cybotes: description and field evidence. Breviora 430:1–9
Williams EE (1977) Additional notes on distichoids. In: Williams EE (ed) The third Anolis newsletter. Museum of Comparative Zoology, Cambridge, pp 174–182
Acknowledgements
We thank Allison Kerwin and Bryan Falk for their help in developing the microsatellite library, as well as Daniel Scantlebury and Shane Campbell-Staton for their comments on this manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ng, J., Perkins, S.L., Dussmann, E.J. et al. Eleven highly polymorphic microsatellite markers for the lizard Anolis distichus . Conservation Genet Resour 1, 135–139 (2009). https://doi.org/10.1007/s12686-009-9033-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-009-9033-8