Abstract
We describe ten microsatellite loci from the lowland tapir (Tapirus terrestris), one of the last members of the Neotropical megafauna and considered a vulnerable species. A total of 74 alleles were detected, ranging from four to thirteen alleles per locus. The expected heterozygosity ranged from 0.34 to 0.88, with a mean of 0.635. All but one locus conformed to the Hardy–Weinberg expectations. These microsatellite loci can be used in population genetic studies of the genus Tapirus, in which all species are threatened globally.
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References
Benjamin Y, Yekutieli D (2001) The control of false discovery rate under dependency. Ann Statist 29:1165–1188
Bodmer RE, Brooks DM (1997) Status and action plan of the lowland tapir (Tapirus terrestris). In: Brooks DM, Bodmer RE, Matola S (eds) Tapirs–Status survey and conservation plan. IUCN/SSC Tapir Specialist Group, IUCN, Gland, Switzerland, pp 46–56
Emmons LH, Feer F (1997) Neotropical rainforest mammals: a field guide, 2nd edn. The University of Chicago Press, Chicago, pp 173–174
Freitas PD, Martins DS, Galetti PM Jr (2008) CID (Clipping vectors, Identifying SSR, Design of primers): a rapid and efficient bioinformatics tool for the detection of simple sequence repeats (SSRs) from genomic libraries. Mol Ecol Resour 8:107–108
Galetti M, Giacomini H, Bueno R, Marques RM, Bernardo CSS, Bovendorp R, Gobbo S, Donatti C, Steffler CE, Meirelles F, Anzolin RB, Nobre R, Chiarello A, Peres CA (2009) Priority areas for the conservation of Atlantic forest large mammals. Biol Conserv 142:1229–1241
Hamilton MB, Pincus EL, Di Fiore A, Fleischer RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. BioTechniques 27:500–507
Hansen DM, Galetti M (2009) The forgotten megafauna. Science 324:42–43
Lahiri DK, Nurnberger JI (1991) A rapid non-enzymatic method for the preparation of HMW DNA from blood for RFLP studies. Nucleic Acids Res 19:5444
Padilha M, Dowler RC (1994) Tapirus terrestris. Mamm Species 481:1–8
Raymond M, Rousset F (1995) GENEPOP (version 1.2): a population genetic software for exact test and ecumenism. J Hered 86:248–249
Schipper J et al (2008) The status of the world’s land and marine mammals: diversity, threat, and knowledge. Science 322:225–230
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234
van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Acknowledgments
We thank to Flávio H. da Silva for facilitating the use of the automated sequencer, Marcelo Cervini for help in genotyping and the zôos for allowing the collection of hair samples (Parque Ecológico Dr. Antonio T. Vianna/São Carlos, Bosque dos Jequitibás/Campinas, Zoo Municipal de Mogi-Mirim, Zoo Municipal de Limeira, Parque Ecológico Isidoro Bordon/Nova Odessa. This work was supported by FAPESP (BIOTA 2007/03392-6, 2007/04073-1), FUNDUNESP Memphis Zoo—TN (USA) and Idea Wild. PMGJ is supported by CNPq.
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Sanches, A., de Figueiredo, M.G., Hatanaka, T. et al. Microsatellite loci isolated from the lowland tapir (Tapirus terrestris), one of the largest Neotropical mammal. Conservation Genet Resour 1, 115–117 (2009). https://doi.org/10.1007/s12686-009-9028-5
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DOI: https://doi.org/10.1007/s12686-009-9028-5