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Isolation and characterization of 13 microsatellite loci in the nine-spined stickleback (Pungitius pungitius) and cross-species amplification in 5 stickleback species (family Gasterosteidae)

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Abstract

A microsatellite-enriched genomic library was obtained from the nine-spined stickleback, the freshwater type of Pungitius pungitius, and 13 dinucleotide markers were successfully isolated and characterized. Cross-species amplification of these markers in three Pungitius species and two Gasterosteus species (family Gasterosteidae) was performed under standard conditions. The mean observed and expected heterozygosity was 0.500–0.688 and 0.508–0.750, respectively. Low allelic diversity was noted among the microsatellite loci in P. tymensis. These results confirm the genetic vulnerability of Pungitius and the need for its conservation. The newly established microsatellite markers will facilitate population-genetic and ecological studies for the conservation of sympatric sticklebacks.

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Acknowledgements

We thank R. Yokoyama and Y. Hashiguchi for their constructive discussions. This work was partly supported by Grants-in-Aid for Young Scientists (B) and Scientific Research (19770074; 19405011; and 19207007) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. Division of Marine Biosciences, Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, 041-8611, Japan

    Yu-ichiro Meguro & Akira Goto

  2. Department of Applied Aquabiology, National Fisheries University, Nagata-honmachi 2-7-1, Shimonoseki, 759-6595, Japan

    Hiroshi Takahashi

  3. National Research Institute of Fisheries Science, Ueda Station, 1081 Komaki, Ueda, Nagano, 386-0031, Japan

    Hirohiko Takeshima

  4. Department of Marine Bioscience, Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Nakano, Tokyo, 164-8639, Japan

    Hirohiko Takeshima & Mutsumi Nishida

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  1. Yu-ichiro Meguro
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  2. Hiroshi Takahashi
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  3. Hirohiko Takeshima
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  4. Mutsumi Nishida
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  5. Akira Goto
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Correspondence to Yu-ichiro Meguro.

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Meguro, Yi., Takahashi, H., Takeshima, H. et al. Isolation and characterization of 13 microsatellite loci in the nine-spined stickleback (Pungitius pungitius) and cross-species amplification in 5 stickleback species (family Gasterosteidae). Conservation Genet Resour 1, 31 (2009). https://doi.org/10.1007/s12686-009-9007-x

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  • DOI: https://doi.org/10.1007/s12686-009-9007-x

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