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Isolation of microsatellites via enrichment and a sequence tagged method in a South American lizard with suspected parental care, Liolaemus leopardinus

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Abstract

We used an enrichment and a sequence tagged microsatellite method (STM) to isolate 11 novel di and tri microsatellites for the high altitude living lizard Liolaemus leopardinus. These loci were trialled in 61 individuals from a single population. Seven loci conformed to Hardy–Weinberg expectations and were in linkage equilibrium. The 7 loci displayed intermediate levels of polymorphism exhibiting between 2 and 10 alleles per locus, with observed heterozygosity values ranging from 0.328 to 0.745. These markers will be useful in population level analyses and in the potential identification of complex social structure and parental care in this species.

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Acknowledgments

We acknowledge the assistance and guidance from researchers at the Evolutionary Biology Unit of the South Australian Museum and Alison Fitch from Biological Science, Flinders University.

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Authors and Affiliations

  1. School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA, 5001, Australia

    Andrew R. Murphy & Michael G. Gardner

  2. Department of Zoology, Oklahoma State University, 430 LSW, Stillwater, OK, 74078, USA

    Stanley F. Fox

Authors
  1. Andrew R. Murphy
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  2. Michael G. Gardner
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  3. Stanley F. Fox
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Correspondence to Michael G. Gardner.

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Murphy, A.R., Gardner, M.G. & Fox, S.F. Isolation of microsatellites via enrichment and a sequence tagged method in a South American lizard with suspected parental care, Liolaemus leopardinus . Conservation Genet Resour 1, 13–16 (2009). https://doi.org/10.1007/s12686-009-9003-1

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  • DOI: https://doi.org/10.1007/s12686-009-9003-1

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