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Oxygen Electroreduction Catalyzed by Bilirubin Oxidase Does Not Release Hydrogen Peroxide

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Abstract

This paper reports on the electroreduction of oxygen catalyzed by bilirubin oxidase (BOD) without H2O2 production. The enzyme was immobilized in a Nafion® matrix in the presence of either a commercial mediator, the 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), or a synthesized osmium redox complex to increase the kinetically slow bio-electroreaction. The use of a rotating ring-disk electrode system demonstrated for the first time the absence of hydrogen peroxide production during the oxygen reduction reaction at the active BOD centers mediated by ABTS. These results were compared with those obtained on Pt-based catalyst. We showed that BOD has the advantage to reduce O2 at a smaller overpotential value (100 mV) than Pt (260 mV).

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Acknowledgments

This work was mainly conducted within the framework of a collaborative program, Programme Hubert Curien—Ulysses n° 21262ZA.

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Authors and Affiliations

  1. Département de Chimie, “Equipe E-lyse” LACCO UMR CNRS 6503, Université de Poitiers, 4 rue Michel Brunet-B27, BP 633, 86022, Poitiers Cedex, France

    Pradel Tonda-Mikiela, Aurélien Habrioux, Karine Servat, Teko W. Napporn & K. Boniface Kokoh

  2. Department of Chemistry, National University of Ireland, Galway, Ireland

    Susan Boland, Paul Kavanagh & Donal Leech

  3. Institut Européen des Membranes, UMR 5635, ENSCM-UMII-CNRS, Place Eugène Bataillon, 34095, Montpellier, France

    Sophie Tingry

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  1. Pradel Tonda-Mikiela
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  2. Aurélien Habrioux
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  3. Susan Boland
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  4. Karine Servat
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  7. Teko W. Napporn
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  9. K. Boniface Kokoh
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Correspondence to K. Boniface Kokoh.

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Tonda-Mikiela, P., Habrioux, A., Boland, S. et al. Oxygen Electroreduction Catalyzed by Bilirubin Oxidase Does Not Release Hydrogen Peroxide. Electrocatal 2, 268–272 (2011). https://doi.org/10.1007/s12678-011-0062-1

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  • DOI: https://doi.org/10.1007/s12678-011-0062-1

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