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Phytotoxicity assessment of γ-Fe2O3 nanoparticles on root elongation and growth of rice plant

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Abstract

This study was conducted to determine the phytotoxicity of 6 nm γ-Fe2O3 nanoparticles (IONPs) in terms of root elongation and the physiological performance of rice plants. Rice seeds (Oryza sativa L. var. Koshihikari) exposed to IONPs at 500, 1,000 and 2,000 mg/L, had a significantly higher root elongation than the control and its bulk counterparts (IOBKs), indicating that the effect can be nanospecific. In a 14-week greenhouse pot experiment, the CO2 assimilation rate in IOBK and IONP-treated pots (500 and 1,000 mg/pot) decreased over time, with the decline (maximum 42.5 %) being less pronounced for IONPs, indicating that the effect cannot be inferred from the toxicity of nanoscale size iron oxide. Excessive adsorption of IONPs onto soil colloids with subsequent low water extractable iron was responsible for the unremarkable phytotoxic nature of IONPs in the rice plants. Amendment of IONPs coated with 20 mmol citric acid (IONPs-Cit) significantly diminished the CO2 assimilation rate and the decrease was similar to its bulk counterpart (IOBKs-Cit). However, maximum shoot growth inhibition (37 %) was associated with the application of IOBKs-Cit. It was concluded that massive accumulations of Fe plaque on the root surfaces of IOBKs-Cit treatments due to a decline in the pH of rhizoplane soils compared to the IONPs-Cit treatments were responsible for the remarkable shoot growth reduction. This study provided evidence of the phytotoxicity of γ-Fe2O3 nanoparticles, demonstrating the lower toxicity of nanosized iron oxide compared to a microsized preparation under reductive conditions.

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Acknowledgments

The authors wish to thank Masanori Hanata and Yosuke Miyauchi for their contribution to the laboratory works as well as for their technical supports. Special thanks go to Dr. Masayuki Kawahigashi from Tokyo Metropolitan University for his kind supports in determination of iron using ICP-AES.

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  1. Department of Environmental Horticulture, Division of Bioresource Science, Laboratory of Crop Science, Chiba University, Matsudo, 648, Japan

    Darioush Alidoust & Akihiro Isoda

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  1. Darioush Alidoust
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  2. Akihiro Isoda
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Correspondence to Darioush Alidoust.

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Alidoust, D., Isoda, A. Phytotoxicity assessment of γ-Fe2O3 nanoparticles on root elongation and growth of rice plant. Environ Earth Sci 71, 5173–5182 (2014). https://doi.org/10.1007/s12665-013-2920-z

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