Abstract
In the international literature, the role of Ozone (O3) in the advancement in alveolar bone healing in the absence of bone pathology was not tested before. The purpose of this study was to evaluate alveolar bone regeneration after a bone defect was created and treated with a single topical administration of O3. Alveolar bone defects were created on five healthy chacma baboons. One side of the maxilla and mandible was topically treated with a single treatment of an O3/O2 mixture (3,5–4 % O3), while the opposite sides were not treated and thus served as control. Regeneration was measured radiologically, using a standardized gray scale, as the increase in bone density in the treatment area at 3 and 6 weeks post-operative and was statistically analyzed using multivariate analysis of variance (MANOVA). There were no significant differences in densities observed between the O3/O2 mixture treatment and the control (p > 0.05). A single O3 treatment did not increase alveolar bone healing over a 3- and 6-week period in the mandible and the maxilla.
Similar content being viewed by others
References
Nogales CG, Ferrari PA, Kantorovich EO, Lage-Marques JL (2008) Ozone therapy in medicine and dentistry. J Contemp Dent Pract 9:75–84
Azarpazhooh A, Limeback H (2008) The application of ozone in dentistry: a systematic review of literature. J Dent 36:104–116
Lynch E (2009) Comment on the application of ozone in dentistry: a systematic review of the literature. J Dent 37:406–410
Bocci V, Lynch E (eds) (2004) Ozone: the revolution in dentistry. Quintessence Publishing Co., London, pp 15–22
Bocci VA (2006) Scientific and medical aspects of ozone therapy. State of the art. Arch Med Res 37:425–435
Stubinger S, Filippi A (2006) The use of ozone in dentistry and maxillofacial surgery: a review. Quintessence Int 37:353–359
Bocci V, Luzzi E, Corradeschi F (2002) Oxygen-ozone therapy, a critical evaluation. Kluwer Academic Publishers, Dordrecht, p 472
Bocci VA (2007) Can ozone therapy be performed if the biochemistry of the process cannot be controlled? Arch Med Res 38:584–585
Bocci V (2004) Ozone as janus: this controversial gas can be either toxic or medically useful. Med Inflamm 13:3–11
Oizumi M, Suzuki T, Uchida M, Furuya J, Okamoto Y (1998) In vitro testing of a denture cleaning method using ozone. J Med Dent Sci 45:135–139
Ripamonti C, Maniezzo M, Ghiringhelli R, Cislaghi E, Mariani L (2009) Medical ozone (O3) oil or gas applications heal osteonecrosis of the jaw (ONJ) in patients treated with bisphosphonates (BPs): preliminary results of a single arm study. Cancer Res 69:5046
Agrillo A, Sassano P, Rinna C, Priore P, Iannetti G (2007) Ozone therapy in extractive surgery on patients treated with bisphosphonates. J Craniofac Surg 18:1068–1070
Agrillo A, Ungari C, Filiaci F, Priore P, Iannetti G (2007) Ozone therapy in the treatment of avascular bisphosphonate-related jaw osteonecrosis. J Craniofac Surg 18:1071–1075
Ripamonti C, Cislaghi E, Massimo LM (2011) Efficacy and safety of medical ozone (O3) delivered in oil suspension applications for the treatment of osteonecrosis of the jaw in patients with bone metastases treated with bisphosphonates: preliminary results of a phase I–II study. Oral Oncol 47:185–190
Daif ET (2012) Role of intra-articular ozone gas injection in the management of internal derangement of the temporomandibular joint. Oral Surg Oral Med Oral Pathol Oral Radiol 113:e10–e14
Petrucci MT, Gallucci C, Agrillo A, Mustazza MA, Robin FO (2007) Role of ozone therapy in the treatment of osteonecrosis of the jaws in multiple myeloma patients. Haematologica 92:1289–1290
Kashperskii YuP, Adamyan AA, Makarov VA, Glyantsev SP, Zhukov VA (1995) Study of the hemostatic properties of gaseous ozone. Eksp Biol Med 120:62–65
Rodriqurz PG, Felix FN, Woodley DT, Shim EK (2008) The role of oxygen in wound healing: a review of the literature. Dermatol Surg 34:1159–1169
Schrem S, Szeimies RM, Prantl L, Karrer S, Landthaler M, Babilas P (2010) Oxygen in acute and chronic wound healing. Br J Dermatol 163:257–268
Bishop A (2008) Role of oxygen in wound healing. J Wound Care 17:399–402
Tandara AA, Mustoe TA (2004) Oxygen in wound healing-more than a nutrient. World J Surg 28:294–300
Du Plessis LH, van der Westhuizen FH, Kotzé HF (2007) The effect of blood ozonation on mitochondrial function and apoptosis of peripheral blood mononuclear cells in the presence and absence of plasma antioxidants. Afr J Biotechnol 6:1763–1769
Kotze MJ, Bütow K-W, Kotze HF (2012) A radiological method to evaluate alveolar bone regeneration in the Chacma baboon (Papio ursinus). SADJ 67:210–214
Bittar-Cortez JA, Passeri LA, Bóscolo FN, Haiter-Neto F (2006) Comparison of hard tissue density changes around implants assessed in digitized conventional radiographs and subtraction images. Clin Oral Implants Res 17:560–564
Breiner MA (2007) Ozone in dentistry. In: Whole body news update Feb 2007. KSB Promotions, Michigan
Frascino AVM, Juliana N, Andrea M, Zindel MC (2010) Bone repair of monocortical wounds after ozonized water irrigation. IADR, Barcelona
Burdurlu Ç, Delibasi Ç, Denize E, Arslan A (2011) Evaluation of effects of topical ozone and chlorhexidine application on alveolar osteitis and wound infection following mandibular impacted third molar surgery. Turkiye Klinikleri J Dental Sci 17:17–23
Broussard CL (2004) Hyperbaric oxygenation and wound healing. J Vasc Nurs 22:42–48
Fries RB, Wallace WA, Roy S, Kuppusamy P, Bergdall V, Gordillo GM, Melvin WS, Sen CK (2005) Dermal excisional wound healing in pigs following treatment with topically applied pure oxygen. Mutat Res 579:172–181
Riva-Sanseverino E (1987) The influence of ozone therapy on the remineralization of bone tissue in osteoporosis. Ozonachrichten 6:75–82
Phillipi A (1999) The use of ozonized water as a cooling and irrigating medium during osteotomy procedures. Community Dent Oral Epidemiol 10:619–922
Filippi A (2001) The influence of ozonised water on the epithelial wound healing process in oral cavity (in German). Dtsch Zahnarztl 56:104–108
Estrela C, Estrela CRA, Decurcio DA, Hollanda ACB, SilvaJ A (2007) Antimicrobial efficacy of ozonated water, gaseous ozone, sodium hypochlorite and chlorhexidine in infected human root canals. Intern Endod J 40:85–93
Hems RS, Gulabivala K, Ng YL, Ready D, Spratt DA (2005) An in vitro evaluation of the ability of ozone to kill a strain of Enterococcus faecalis. Intern Endod J 38:22–29
Nagayoshi M, Kitamura C, Fukuizumi T, Nishihara T, Terashita M (2004) Antimicrobial effect of ozonated water on bacteria invading dentinal tubules. J Endod 30:778–781
Aguirre JI, Altman MK, Vanegas SM, Franz SE, Bassit ACF (2010) Effects of alendronate on bone healing after tooth extraction in rats. Oral Dis 16:674–685
Acknowledgments
The personnel of the Animal Facility of the North-West University supplied the animals; Unique Dental, South Africa, supplied the radiological equipment and computer software and the Dental Research Education & Development Trust of the South African Dental Association contributed financially. Permission was granted by the Managing Editor of The South African Dental Journal for the use of figures. (Kotze MJ, et al. SADJ 67:210–214)
Conflict of interest
None.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kotze, M., Bütow, KW., Olorunju, S.A. et al. Ozone Treatment of Alveolar Bone in the Cape Chacma Baboon Does Not Enhance Healing Following Trauma. J. Maxillofac. Oral Surg. 13, 140–147 (2014). https://doi.org/10.1007/s12663-013-0471-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12663-013-0471-1