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Visualization of respiratory flows from 3D reconstructed alveolar airspaces using X-ray tomographic microscopy

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Abstract

A deeper knowledge of the three-dimensional (3D) structure of the pulmonary acinus has direct applications in studies on acinar fluid dynamics and aerosol kinematics. To date, however, acinar flow simulations have been often based on geometrical models inspired by morphometrical studies; limitations in the spatial resolution of lung imaging techniques have prevented the simulation of acinar flows using 3D reconstructions of such small structures. In the present study, we use high-resolution, synchrotron radiation-based X-ray tomographic microscopy (SRXTM) images of the pulmonary acinus of a mouse to reconstruct 3D alveolar airspaces and conduct computational fluid dynamic (CFD) simulations mimicking rhythmic breathing motion. Respiratory airflows and Lagrangian (massless) particle tracking are visualized in two examples of acinar geometries with varying size and complexity, representative of terminal sacculi including their alveoli. The present CFD simulations open the path towards future acinar flow and aerosol deposition studies in complete and anatomically realistic multi-generation acinar trees using reconstructed 3D SRXTM geometries.

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References

  • Ardila R, Horie T, Hildebrandt J (1974) Macroscopic isotropy of lung expansion. J Appl Physiol 20:105–115

    Google Scholar 

  • Aykac D, Hoffman EA (2003) Segmentation and analysis of the human airway tree from three-dimensional X-ray CT images. IEEE Trans Med Imaging 22:940–950

    Article  Google Scholar 

  • Bonara M, Bernaudin JF, Guernier C, Brahimi-Horn MC (2004) Ventilatory responses to hypercapnia and hypoxia in conscious cystic fibrosis knockout mice Cftr−/−. Pediatr Res 55:738–746

    Article  Google Scholar 

  • Brown RH, Herold CJ et al (1991) In vivo measurements of airway reactivity using high resolution computed tomography. Am Rev Respir Dis 144:208–212

    Google Scholar 

  • Brunekreef B, Holgate ST (2002) Air pollution and health. Lancet 360:1233–1242

    Article  Google Scholar 

  • Burri PH, Dbaly J, Weibel ER (1974) The postnatal growth of the rat lung. I. Morphometry. Anat Rec 178:711–730

    Article  Google Scholar 

  • Darmofal DL, Haimes R (1996) An analysis of 3D particle path integration algorithms. J Comp Phys 123:182–195

    Article  MATH  Google Scholar 

  • Darquenne C (2001) A realistic two-dimensional model of aerosol transport and deposition in the alveolar zone of the human lung. J Aerosol Sci 32:1161–1174

    Article  Google Scholar 

  • Ferziger JH, Peric M (2001) Computational methods for fluid dynamics, 3rd edn. Springer, Berlin

    Google Scholar 

  • Fung YC (1988) A model of the lung structure and its validation. J Appl Physiol 64:2132–2141

    Google Scholar 

  • Gil J, Bachofen H, Gehr P, Weibel ER (1979) Alveolar volume-surface area relation in air- and saline-filled lungs fixed by vascular perfusion. J Appl Physiol 45:990–1001

    Google Scholar 

  • Haas CS, Amann K, Schittny JC et al (2003) Glomerular and renal vascular structural changes in alpha 8 integrin-deficient mice. J Am Soc Nephrol 14:2288–2296

    Article  Google Scholar 

  • Haber S, Tsuda A (1998) The effect of flow generated by a rhythmically expanding pulmonary acinus on aerosol dynamics. J Aerosol Sci 29:309–322

    Article  Google Scholar 

  • Haber S, Yitzhak D, Tsuda A (2003) Gravitational deposition in a rhythmically expanding and contracting alveolus. J Appl Physiol 95:657–671

    Google Scholar 

  • Haefeli-Bleuer B, Weibel ER (1988) Morphometry of the human pulmonary acinus. Anat Rec 220:401–414

    Article  Google Scholar 

  • Hansen JE, Ampaya EP (1975) Human air space shape, sizes, areas and volumes. J Appl Physiol 38:990–995

    Google Scholar 

  • Hansen JE, Ampaya EP, Bryant GH, Navin JJ (1975) Branching pattern of airways and air spaces of a single human terminal bronchiole. J Appl Physiol 38:983–989

    Google Scholar 

  • Henry FS, Butler JP, Tsuda A (2002) Kinematically irreversible acinar flow: a departure from classical dispersive aerosol transport theories. J Appl Physiol 92:835–845

    Google Scholar 

  • Kitaoka H, Tamura S, Takaki R (2000) A three-dimensional model of the human pulmonary acinus. J Appl Physiol 88:2260–2268

    Google Scholar 

  • Luyet C, Burri PH, Schittny JC (2002) Suppression of cell proliferation and programmed cell death by dexamethasone during postnatal lung development. Am J Physiol Lung Cell Mol Physiol 282:L477–L483

    Google Scholar 

  • McNamara AE, Muller NL, Okazawa M et al (1992) Airway narrowing in excised canine lungs measured by high-resolution computed tomography. J Appl Physiol 73:307–316

    Google Scholar 

  • Mercer RR, Crapo JD (1987) Three-dimensional reconstruction of the rat acinus. J Appl Physiol 63:785–794

    Google Scholar 

  • Muhlfeld C, Rothen-Ruthishauser B, Blank F et al (2008) Interactions of nanoparticles with pulmonary structures and cellular responses. Am J Physiol Lung Cell Mol Physiol 294:L817–L829

    Article  Google Scholar 

  • Mund SI, Stampanoni M, Schittny JC (2008) Developmental alveolarization of the mouse lung. Dev Dyn 237:2108–2116

    Article  Google Scholar 

  • Pokorski M, Izumizaki M, Homma I (2005) Transient O2-dependent effect of CO2 on ventilation in the anesthetized mouse. J Physiol Pharmacol 56:447–454

    Google Scholar 

  • Roth-Kleiner M, Berger TM, Tarek MR et al (2005) Neonatal Dexamethasone induces premature microvascular maturation by focal fusion of alveolar capillaries. Dev Dyn 233:1261–1271

    Article  Google Scholar 

  • Sauret V, Halson PM, Brown IW et al (2002) Study of the three-dimensional geometry of the central conducting airways in man using computed tomographic (CT) images. J Anat 200:123–134

    Article  Google Scholar 

  • Schittny JC, Mund SI, Stampanoni M (2008) Evidence and structural mechanism for late lung alveolarization. Am J Physiol Lung Cell Mol Physiol 294:L246–L254

    Article  Google Scholar 

  • Sera T, Fujioka H, Yokota H et al (2003) Three-dimensional visualization and morphometry of small airways from microfocal X-ray computed tomography. J Biomech 36:1587–1594

    Article  Google Scholar 

  • Shah RK, London AL (1978) Laminar flow forced convection in ducts. Academic, New York

    Google Scholar 

  • Stampanoni M, Borchet G, Wyss P et al (2002) High resolution X-ray detector for synchrotron based microtomography. Nucl Instr Meth A 491:291–301

    Article  Google Scholar 

  • Sznitman J, Heimsch F, Heimsch T et al (2007) Three-dimensional convective alveolar flow induced by rhythmic breathing motion of the pulmonary acinus. ASME J Biomech Eng 129:658–665

    Article  Google Scholar 

  • Sznitman J, Heimsch T, Wildhaber JH et al (2009) Respiratory flow phenomena and gravitational sedimentation in a three-dimensional space-filling model of the pulmonary acinar tree. ASME J Biomech Eng 131:031010

    Article  Google Scholar 

  • Tsuda A, Filipovic N, Haberthuer D et al (2008) Finite element 3D reconstruction of the pulmonary acinus imaged by synchrotron X-ray tomography. J Appl Physiol 105:964–976

    Article  Google Scholar 

  • Tsuda A, Henry FS, Butler JP (1995) Chaotic mixing of alveolated duct flow in rhythmically expanding pulmonary acinus. J Appl Physiol 79:1055–1063

    Google Scholar 

  • Weibel ER, Knight BW (1964) A morphometric study on the thickness of the pulmonary air–blood barrier. J Cell Biol 21:367–384

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ, 08544, USA

    J. Sznitman

  2. Swiss Light Source Project, Paul Scherrer Institute, 5232, Villigen, Switzerland

    M. Stampanoni

  3. Institute of Biomedical Engineering, ETH Zurich, 8092, Zurich, Switzerland

    M. Stampanoni

  4. Institute of Fluid Dynamics, ETH Zurich, 8092, Zurich, Switzerland

    R. Sutter, D. Altorfer & T. Rösgen

  5. Institute of Anatomy, University of Bern, 3010, Bern, Switzerland

    J. C. Schittny

Authors
  1. J. Sznitman
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  2. R. Sutter
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  3. D. Altorfer
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  4. M. Stampanoni
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  5. T. Rösgen
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  6. J. C. Schittny
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Correspondence to J. Sznitman.

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Sznitman, J., Sutter, R., Altorfer, D. et al. Visualization of respiratory flows from 3D reconstructed alveolar airspaces using X-ray tomographic microscopy. J Vis 13, 337–345 (2010). https://doi.org/10.1007/s12650-010-0043-0

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  • DOI: https://doi.org/10.1007/s12650-010-0043-0

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