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Metabolites from the Mucus of Volutharpa ampullacea perryi: A Prospective Marine Resource for Bioactive Molecules

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Abstract

The aim of this study is to define compositions and bioactive substances in the mucus derived from the deep-sea snail Volutharpa ampullacea perryi. V. ampullacea perryi, the so-called “false abalone,” is a popular inexpensive seafood along the northern coast of China. The mucus secreted by the pleopod gland of this animal has always been considered a waste product. Here, we used traditional biochemical methods, MTT assays, and metabolomics analyses to determine the composition of the mucus and explore its potential value as a source of bioactive molecules. Crude protein was the major nutrient contained in the mucus of V. ampullacea perryi, and these proteins had anti-cancer potential. In addition, we identified and annotated 332 metabolites in the mucus. Of these, 61.75% had pharmacological functions, 3.61% could be used as cosmetics additives, and 9.04% had nutritional value.

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Acknowledgements

This study was financially supported by the Science and Technology Innovation Foundation Project of Dalian (No. 2018J11CY029).

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Author notes

  1. Tanjun Zhao, Simeng Chen, Cong Li and Yaqing Chang have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, Liaoning, People’s Republic of China

    Tanjun Zhao, Senrong Han, Li Liu, Yaoyao Zhan & Yaqing Chang

  2. College of Basic Medical Science, Dalian Medical University, Dalian, 116044, Liaoning, People’s Republic of China

    Simeng Chen, Cong Li & Yujie Jia

Authors
  1. Tanjun Zhao
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  2. Simeng Chen
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  3. Cong Li
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  4. Senrong Han
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  5. Yujie Jia
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  6. Li Liu
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  7. Yaoyao Zhan
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  8. Yaqing Chang
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Contributions

YYZ and YQC conceived and designed the experiments. TJZ, SMC, CL, SRH, and LL performed the experiments. YYZ, TJZ, CL, YJJ and YQC analyzed the data. YYZ, TJZ and SMC wrote the paper. All authors read and approved the manuscript.

Corresponding author

Correspondence to Yaoyao Zhan.

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The authors declare no competing financial interests.

Ethical Approval

The animals used in this study are unregulated invertebrates. Animal welfare and experimental procedures were carried out in accordance with the Laboratory animals-General requirements for animal experiment (National Standards of P. R. China, GB/T 35823—2018).

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Zhao, T., Chen, S., Li, C. et al. Metabolites from the Mucus of Volutharpa ampullacea perryi: A Prospective Marine Resource for Bioactive Molecules. Waste Biomass Valor 12, 4287–4298 (2021). https://doi.org/10.1007/s12649-020-01308-7

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  • DOI: https://doi.org/10.1007/s12649-020-01308-7

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