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Potential Application of a Pseudomonas geniculata ATCC 19374 and Bacillus cereus EC3 Mixture in Livestock Wastewater Treatment

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Abstract

In this study, we isolated two bacterial strains, P2 (Pseudomonas geniculata ATCC 19374, P. geniculata ATCC 19374) and B1 (Bacillus cereus EC3, B. cereus EC3) from pig farm soil, which were mixed to construct a novel bacterial mixture (P2:B1 = 1:1). We studied the heterotrophic nitrification capablity and growth characteristics of bacterial mixture in heterotrophic nitrification medium (HNM). And the factors affecting the nitrogen removal of the bacterial mixture such as C/N ratio, NH4+-N load and inoculation size of the bacterial mixture were investigated in this study. Furthermore, we used the bacterial mixture to treat livestock wastewater to remove NH4+-N, TN and COD. The results showed that the optimal C/N ratio and inoculation size for the bacterial mixture were 12 and 5%, respectively. It is worth to mention that the bacterial mixture shows excellent NH4+-N removal ability under low C/N ratio, indicating that the bacterial mixture had the potential to be applied to wastewater with C/N lower than 8. When the initial NH4+-N concentration was 500 mg/L, the NH4+-N removal efficiency exceeded 90% with a 5% inoculation size of the bacterial mixture after 72 h. When the single bacteria and bacterial mixture were employed to treat livestock wastewater, the removal efficiencies of bacterial mixture on NH4+-N, TN and COD within 72 h were 70.06%, 58.12% and 81.48%, respectively, which were higher than that of single bacteria. The bacterial mixture has potential application value in enhancing the ability of reactors and constructed wetlands to remove pollutants from livestock wastewater and developing a novel and efficient biological nitrogen removal technology. Our current research provides a research basis for the application of the bacterial mixture in the future.

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References

  1. Zhao, B.W., Li, J.Z., Leu, S.Y.: An innovative wood-chip-framework soil infiltrator for treating anaerobic digested swine wastewater and analysis of the microbial community. Bioresour. Technol. 173, 384–391 (2014)

    Google Scholar 

  2. Su, J.J., Liu, B.Y., Chang, Y.C.: Emission of greenhouse gas from livestock waste and wastewater treatment in Taiwan. Agric. Ecosyst. Environ. 95(1), 253–263 (2003)

    Google Scholar 

  3. Jia, S.Y., Zhang, X.X., Miao, Y., Zhao, Y.T., Ye, L., Li, B., Zhang, T.: Fate of antibiotic resistance genes and their associations with bacterial community in livestock breeding wastewater and its receiving river water. Water Res. 124, 259–268 (2017)

    Google Scholar 

  4. Restu, A.A., Erwanto, Y., Hadisaputro, W., Yusiati, L.M., Fitriyanto, N.A.: Characteristics of Alcaligenes sp. LS2T heterotrophic and aerobic ammonium removal for potential livestock’s wastewater treatment. In: Proceeding of the 1st International Conference on Tropical Agriculture 337–344 (2017)

  5. Cho, J.C., Cho, H.B., Kim, S.J.: Heavy contamination of a subsurface aquifer and a stream by livestock wastewater in a stock farming area, Wonju, Korea. Environ. Pollut. (Barking, Essex: 1987) 109(1), 137–146 (2000)

    Google Scholar 

  6. Sasaki, H., Nonaka, J., Sasaki, T., Nakai, Y.: Ammonia removal from livestock wastewater by ammonia-assimilating microorganisms immobilized in polyvinyl alcohol. J Ind. Microbiol. Biot. 34(2), 105–110 (2007)

    Google Scholar 

  7. Sun, S.P., Nàcher, C.P.I., Merkey, B., Zhou, Q., Xia, S.Q., Yang, D.H., Sun, J.H., Smets, B.F.: Effective biological nitrogen removal treatment processes for domestic wastewaters with low C/N ratios: a review. Environ. Eng. Sci. 27(2), 111–126 (2010)

    Google Scholar 

  8. Khardenavis, A.A., Kapley, A., Purohit, H.J.: Simultaneous nitrification and denitrification by diverse Diaphorobacter sp. Appl. Microbiol. Biotechnol. 77(2), 403–409 (2007)

    Google Scholar 

  9. Khin, T., Annachhatre, A.P.: Novel microbial nitrogen removal processes. Biotechnol. Adv. 22(7), 519–532 (2004)

    Google Scholar 

  10. Guštin, S., Marinšek-Logar, R.: Effect of pH, temperature and air flow rate on the continuous ammonia stripping of the anaerobic digestion effluent. Process Saf. Environ. Prot. 89(1), 61–66 (2011)

    Google Scholar 

  11. Luo, X., Su, J., Shao, P., Liu, H., Luo, X.: Efficient autotrophic denitrification performance through integrating the bio-oxidation of Fe(II) and Mn(II). Chem. Eng. 348, 669–677 (2018)

    Google Scholar 

  12. Zhang, J., Wu, P., Hao, B., Yu, Z.: Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001. Bioresour. Technol. 102, 9866–9869 (2011)

    Google Scholar 

  13. Chen, M.X., Wang, W.C., Feng, Y., Zhu, X.H., Zhou, H.Z., Tan, Z.L., Li, X.D.: Impact resistance of different factors on ammonia removal by heterotrophic nitrification-aerobic denitrification bacterium Aeromonas sp HN-02. Bioresour. Technol. 167, 456–461 (2014)

    Google Scholar 

  14. Song, Z.F., An, J., Fu, G.H., Yang, X.L.: Isolation and characterization of an aerobic denitrifying Bacillus sp. YX-6 from shrimp culture ponds. Aquaculture 319(1–2), 188–193 (2011)

    Google Scholar 

  15. Zhang, Q.L., Liu, Y., Ai, G.M., Miao, L.L., Zheng, H.Y., Liu, Z.P.: The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, Bacillus methylotrophicus strain L7. Bioresour. Technol. 108, 35–44 (2012)

    Google Scholar 

  16. Barman, P., Kati, A., Mandal, A.K., Bandyopadhyay, P., Mohapatra, P.K.D.: Biopotentiality of Bacillus cereus PB45 for nitrogenous waste detoxification in ex situ model. Aquacult. Int. 25(3), 1167–1183 (2017)

    Google Scholar 

  17. Shoda, M., Ishikawa, Y.: Heterotrophic nitrification and aerobic denitrification of high-strength ammonium in anaerobically digested sludge by Alcaligenes faecalis strain No. 4. J Biosci. Bioeng. 117(6), 737–741 (2014)

    Google Scholar 

  18. Yan, X., Zheng, J.X., Han, Y.P., Liu, J.W., Sun, J.H.: Effect of influent C/N ratio on N2O emissions from anaerobic/anoxic/oxic biological nitrogen removal processes. Environ. Sci. Pollut. Res. 24(30), 23714–23724 (2017)

    Google Scholar 

  19. Liang, Q., Zhang, X.P., Lee, K.H., Wang, Y.B., Yu, K., Shen, W.Y., Fu, L.Q., Shu, M.A., Li, W.F.: Nitrogen removal and water microbiota in grass carp culture following supplementation with Bacillus licheniformis BSK-4. World J Microb. Biot. 31(11), 1711–1718 (2015)

    Google Scholar 

  20. Joo, H., Hirai, M., Shoda, M.: Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis No. 4. J Biosci. Bioeng. 100(2), 184–191 (2005)

    Google Scholar 

  21. Su, J.F., Zhang, K., Huang, T.L., Wen, G., Guo, L., Yang, S.F.: Heterotrophic nitrification and aerobic denitrification at low nutrient conditions by a newly isolated bacterium, Acinetobacter sp. SYF26. Microbiology 161(4), 829–837 (2015)

    Google Scholar 

  22. Kim, J.K., Park, K.J., Cho, K.S., Nam, S.W., Park, T.J., Bajpai, R.: Aerobic nitrification-denitrification by heterotrophic Bacillus strains. Bioresour. Technol. 96, 1897–1906 (2005)

    Google Scholar 

  23. Yang, J.R., Wang, Y., Chen, H., Lyu, Y.K.: Ammonium removal characteristics of an acid-resistant bacterium Acinetobacter sp. JR1 from pharmaceutical wastewater capable of heterotrophic nitrification-aerobic denitrification. Bioresour. Technol. 274, 56–64 (2019)

    Google Scholar 

  24. Liu, Y., Ai, G.M., Miao, L.L., Liu, Z.P.: Marinobacter strain NNA5, a newly isolated and highly efficient aerobic denitrifier with zero N2O emission. Bioresour. Technol. 206, 9–15 (2016)

    Google Scholar 

  25. Xia, L., Li, X.M., Fan, W.H., Wang, J.L.: Heterotrophic nitrification and aerobic denitrification by a novel Acinetobacter sp. ND7 isolated from municipal activated sludge. Bioresour. Technol. 301, 122749 (2020)

    Google Scholar 

  26. Li, J.Z., Meng, J., Li, J.L., Wang, C., Deng, K.W., Sun, K., Buelna, G.: The effect and biological mechanism of COD/TN ratio on nitrogen removal in a novel upflow microaerobic sludge reactor treating manure-free piggery wastewater. Bioresour. Technol. 209, 360–368 (2016)

    Google Scholar 

  27. Zhao, B., Tian, M., An, Q., Ye, J., Guo, J.S.: Characteristics of a heterotrophic nitrogen removal bacterium and its potential application on treatment of ammonium-rich wastewater. Bioresour. Technol. 226, 46–54 (2016)

    Google Scholar 

  28. Gao, J.Q., Zhu, T.D., Liu, C., Zhang, J.S., Li, Y.: Ammonium removal characteristics of heterotrophic nitrifying bacterium Pseudomonas stutzeri GEP-01 with potential for treatment of ammonium-rich wastewater. Bioprocess Biosyst. Eng. 43(1), 1–11 (2020)

    Google Scholar 

  29. Ni, S.Q., Ni, J.Y., Hu, D.L., Sung, S.W.: Effect of organic matter on the performance of granular anammox process. Bioresour. Technol. 110, 701–705 (2012)

    Google Scholar 

  30. Duan, J.M., Fang, H.D., Su, B., Chen, J.F., Lin, J.M.: Characterization of a halophilic heterotrophic nitrification-aerobic denitrification bacterium and its application on treatment of saline wastewater. Bioresour. Technol. 179, 421–428 (2015)

    Google Scholar 

  31. Zhang, N., Chen, H., Lyu, Y.K., Wang, Y.: Nitrogen removal by a metal-resistant bacterium, Pseudomonas putida ZN1, capable to heterotrophic nitrification-aerobic denitrification. J. Chem. Technol. Biotechnol. 94, 1165–1175 (2018)

    Google Scholar 

  32. Yang, L., Ren, Y.X., Liang, X., Zhao, S.Q., Wang, J.P., Xia, Z.H.: Nitrogen removal characteristics of a heterotrophic nitrifier Acinetobacter junii YB and its potential application for the treatment of high-strength nitrogenous wastewater. Bioresour. Technol. 193, 227–233 (2015)

    Google Scholar 

  33. Liu, Y.X., Hu, T.T., Song, Y.J., Chen, H.P., Lv, Y.K.: Heterotrophic nitrogen removal by Acinetobacter sp. Y1 isolated from coke plant wastewater. J. Biosci. Bioeng. 120, 549–554 (2015)

    Google Scholar 

  34. Zhao, K., Tian, X.L., Li, H.D., Dong, S.L., Jiang, W.W.: Characterization of a novel marine origin aerobic nitrifying-denitrifying bacterium isolated from shrimp culture ponds. Aquac. Res. 8, 1770–1781 (2019)

    Google Scholar 

  35. Joo, H., Hirai, M., Shoda, M.: Improvement in ammonium removal efficiency in wastewater treatment by mixed culture of Alcaligenes faecalis no. 4 and L1. J. Biosci. Bioeng. 103, 66–73 (2007)

    Google Scholar 

  36. Zhang, H.H., Zhao, Z.F., Li, S.L., Chen, S.N., Huang, T., Li, N., Yang, S.Y., Wang, Y., Kou, L.Q., Zhang, X.Y.: Nitrogen removal by mix-cultured aerobic denitrifying bacteria isolated by ultrasound: performance, co-occurrence pattern and wastewater treatment. Chem. Eng. 372, 26–36 (2019)

    Google Scholar 

  37. Kim, M., Jeong, S.Y., Yoon, S.J., Cho, S.J., Kim, Y.H., Kim, M.J., Ryu, E.Y., Lee, S.J.: Aerobic denitrification of Pseudomonas putida AD-21 at different C/N ratios. J. Biosci. Bioeng. 106(5), 498–502 (2008)

    Google Scholar 

  38. Zhang, W., Yan, C., Shen, J.L., Wei, R.P., Gao, Y., Miao, A.J., Xiao, L., Yang, L.Y.: Characterization of aerobic denitrifying bacterium Pseudomonas mendocina strain GL6 and Its potential application in wastewater treatment plant effluent. Int. J. Environ. Res. Public Health 16(3), 364 (2019)

    Google Scholar 

  39. Zhang, Q.Y., Yang, P., Liu, L.S., Liu, Z.J.: Formulation and characterization of a heterotrophic nitrification-aerobic denitrification synthetic microbial community and its application to livestock wastewater treatment. Water 12(1), 218 (2020)

    Google Scholar 

  40. Zhang, D.Y., Li, W.G., Huang, X.F., Qin, W., Liu, M.: Removal of ammonium in surface water at low temperature by a newly isolated Microbacterium sp. strain SFA13. Bioresour. Technol. 137, 147–152 (2013)

    Google Scholar 

  41. Song, Y.J., Yi, L., Liu, Y.X., He, W.L.: Effect of carbon and nitrogen sources on nitrogen removal by a heterotrophic nitrification-aerobic denitrification strain Y1. Acta Sci. Circum. 33(9), 2491–2497 (2013)

    Google Scholar 

  42. Qiao, Z.X., Sun, R., Wu, Y.G., Hu, S.H., Liu, X.Y., Chan, J.W.: Microbial heterotrophic nitrification-aerobic denitrification dominates simultaneous removal of aniline and ammonium in aquatic ecosystems. Water Air Soil Pollut. 231(3), 1–14 (2020)

    Google Scholar 

  43. Rout, P.R., Bhunia, P., Dash, R.R.: Simultaneous removal of nitrogen and phosphorous from domestic wastewater using Bacillus cereus GS-5 strain exhibiting heterotrophic nitrification, aerobic denitrification and denitrifying phosphorous removal. Bioresour. Technol. 244, 484–495 (2017)

    Google Scholar 

  44. Zhao, B., Ran, X.C., Tian, M., An, Q., Guo, J.S.: Assessing the performance of a sequencing batch biofilm reactor bioaugmented with P. stutzeri strain XL-2 treating ammonium-rich wastewater. Bioresour. Technol. 270, 70–79 (2018)

    Google Scholar 

  45. Vadivelu, V.M., Keller, J., Yuan, Z.: Effect of free ammonia on the respiration and growth processes of an enriched Nitrobacter culture. Water Res. 41(4), 826–834 (2007)

    Google Scholar 

  46. Ramadan, M.A., El-Tayeb, O.M., Alexander, M.: Inoculum size as a factor limiting success of inoculum for biodegradation. Appl. Environ. Microbiol. 56(5), 1392–1396 (1990)

    Google Scholar 

  47. Zhao, B., Ye, J., An, Q., Lv, Q., Chen, J.: Optimization of cultivating conditions for ammonium removal by Alcaligenes sp. HN in synthetic ammonium-containing wastewater. Disaster Adv. 6, 308–315 (2013)

    Google Scholar 

  48. Barman, P., Bandyopadhyay, P., Kati, A., Paul, T., Mandal, A.K., Mondal, K.C., Das Mohapatra, P.K.: Characterization and strain improvement of aerobic denitrifying EPS producing bacterium Bacillus cereus PB88 for shrimp water quality management. Waste Biomass Valoriz. 9, 1319–1330 (2018)

    Google Scholar 

  49. Yang, Y.L., Lin, E., Sun, S.Q., Tao, X.Y., Zhong, L., Hu, K.H.: Piggery wastewater treatment by Acinetobacter sp. TX5 immobilized with spent mushroom substrate in a fixed-bed reactor. Sci. Total Environ. 644, 1460–1468 (2018)

    Google Scholar 

  50. Chen, L.F., Lin, J.Q., Pan, D., Ren, Y.L., Lin, J.Q.: Ammonium removal by a newly isolated heterotrophic nitrification-aerobic denitrification bacteria Pseudomonas Stutzeri SDU10 and its potential in treatment of piggery wastewater. Curr. Microbiol. 77(7), 2792–2801 (2020)

    Google Scholar 

  51. Yu, Y., An, Q., Zhou, Y., Deng, S.M., Miao, Y., Zhao, B., Yang, L.: Highly synergistic effects on ammonium removal by the co-system of Pseudomonas stutzeri XL-2 and modified walnut shell biochar. Bioresour. Technol. 280, 239–246 (2019)

    Google Scholar 

  52. Bruger, E.L., Watersa, B.C.M.: Maximizing growth yield and dispersal via quorum sensing promotes cooperation in Vibrio bacteria. Appl. Environ. Microbiol. 84(14), 402–418 (2018)

    Google Scholar 

  53. Patel, A.B., Mahala, K., Jain, K., Madamwar, D.: Development of mixed bacterial cultures DAK11 capable for degrading mixture of polycyclic aromatic hydrocarbons (PAHs). Bioresour. Technol. 253, 288–296 (2018)

    Google Scholar 

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Acknowledgements

The authors are thankful for the support by the Major Special Science and Technology Project of Henan Province (181100310300), the National Science and Technology Major Project (2017ZX07602-003-002), and the fellowship of China Postdoctoral Science Foundation (2020TQ0284).

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Authors and Affiliations

  1. School of Ecology and Environment, Zhengzhou University, Zhengzhou, 450001, Henan, People’s Republic of China

    Lina Liu, Jingqing Gao & Jianlei Gao

  2. School of Water Conservancy and Engineering, Zhengzhou University, Zhengzhou, 450001, People’s Republic of China

    Zhenzhen Huang

  3. Zhengzhou Yuanzhihe Environmental Protection Technology Co., Ltd., Zhengzhou, 450000, People’s Republic of China

    Yonghong Li

  4. School of Pharmaccutical Sciences, Zhengzhou University, Zhengzhou, 450001, People’s Republic of China

    Na Shang

  5. Yellow River Engineering Consulting Co., Ltd., Zhengzhou, 450003, People’s Republic of China

    Jinliang Zhang & Ming Cai

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  1. Lina Liu
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Liu, L., Gao, J., Huang, Z. et al. Potential Application of a Pseudomonas geniculata ATCC 19374 and Bacillus cereus EC3 Mixture in Livestock Wastewater Treatment. Waste Biomass Valor 12, 3927–3938 (2021). https://doi.org/10.1007/s12649-020-01286-w

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