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Enzymatic Production of Xylooligosaccharides from Brown Coconut Husk Treated with Sodium Hydroxide

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Abstract

The present study demonstrates the application of alkali treatment coupled with steam for the extraction of xylan followed by its enzymatic hydrolysis for the production of xylooligosaccharide (XOS) from coconut husk. The chemical analysis of the husk revealed the presence of hemicelluloses, along with cellulose, lignin and ash demonstrating its suitability for XOS extraction. Application of 20% of NaOH coupled with steam treatment for 60 min enabled the fractionation of approximately 93% xylan from the husk. Response surface methodology was employed to optimize the different variables i.e. enzyme concentration, pH, temperature and time for the extraction of XOS from xylan, wherein, enzyme concentration and temperature were the influential variables affecting xylose yield and enzyme concentration, temperature, time and pH were influential variables affecting xylobiose yield significantly. FTIR analysis of the husk also confirmed the presence of hemicelluloses, whereas, the presence of xylan and XOSmix was also confirmed in the extracted mass. In nutshell, the present study was successful in establishing the suitability of coconut husk for extraction of xylan and XOS which can be further used as a prebiotic.

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Acknowledgements

The authors are thankful to the Department of Food Technology and Nutrition (School of Agriculture) and School of Basic Sciences, Lovely Professional University, Phagwara (Punjab) for providing infrastructural facilities and financial support.

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Authors and Affiliations

  1. Food Technology and Nutrition, School of Agriculture, Lovely Professional University, Phagwara, Punjab, 144411, India

    Prakriti Jnawali, Vikas Kumar & Prerna Gupta

  2. Department of Dairy Technology, Mansinhbhai Institute of Dairy and Food Technology, Mehsana, Gujarat, 384002, India

    Beenu Tanwar

  3. Faculty of Family and Community Sciences, M.S. University of Baroda, Sayajibaug Main Road, Opp. Sayajibaug, Pratapgunj, Baroda, 390002, India

    Harsha Hirdyani

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  1. Prakriti Jnawali
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Correspondence to Vikas Kumar.

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Jnawali, P., Kumar, V., Tanwar, B. et al. Enzymatic Production of Xylooligosaccharides from Brown Coconut Husk Treated with Sodium Hydroxide. Waste Biomass Valor 9, 1757–1766 (2018). https://doi.org/10.1007/s12649-017-9963-4

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